华中地区供水水库抗生素抗性基因的季节变化及影响因素  

Seasonal Variation and Influencing Factor Analysis of Antibiotic Resistance Genes in Water Supply Reservoirs of Central China

作　　者：张凯 辛蕊 李贶家 王倩 王亚南 许智恒 崔向超 魏巍 ZHANG Kai;XIN Rui;LI Kuang-jia;WANG Qian;WANG Ya-nan;XU Zhi-heng;CUI Xiang-chao;WEI Wei(Henan Key Laboratory for Synergistic Prevention of Water and Soil Environmental Pollution,School of Geographic Sciences,Xinyang Normal University,Xinyang 464000,China;School of Marine Science and Technology,Tianjin University,Tianjin 300350,China;Development Research Center,Ministry of Water Resources,Beijing 100038,China)

机构地区：[1]信阳师范学院地理科学学院,河南省水土污染协同防治重点实验室,信阳464000 [2]天津大学海洋科学与技术学院,天津300350 [3]水利部发展研究中心,北京100038

出　　处：《环境科学》2021年第10期4753-4760,共8页Environmental Science

基　　金：国家自然科学基金项目(42007209,51808477,41807038);信阳师范学院“南湖学者奖励计划”青年项目。

摘　　要：为明确华中地区饮用水水源地抗生素抗性基因(antibiotic resistance genes,ARGs)的赋存特征,本研究选取了11个大型饮用水水源地,分别于2019年秋季和2020年夏季对整合子基因intI1和19个ARGs进行了定量.结果表明,水库环境中ARGs的丰度并不随时间产生明显变化.四环素类、磺胺类及β-内酰胺类耐药基因是华中地区供水水库的优势基因.选取的2种磺胺类ARGssul1及sul2丰度和检出率均处于较高水平,表明这两种基因是水库环境的优势基因.耐多粘菌素基因mcr-1并未检出,表明我国抗生素限用政策已取得一定效果.与其他环境介质相比,水库环境中ARGs丰度处于较低水平.相关性分析结果显示水质指标与ARGs有一定相关性,表明水质指标可以成为水库环境中ARGs污染的指示指标.由于碳青霉烯类抗生素用量受到严格限制及其易降解的特性,其相应ARGs丰度及检出率均较低;四环素类耐药基因与其他类耐药基因关系密切,基因的水平转移可能是导致该现象的重要因素;intI1虽然整体上与ARGs相关性不强,但仍可能是导致水库环境中个别基因传播扩散的重要因素.This study quantified an integron gene intll and 19 antibiotic resistance genes(ARGs)to identify the ARGs pollution characteristics in 11 drinking water reservoirs of central China.The results indicated that the ARGs abundance did not change significantly over time in the studied reservoir waterbodies.Tetracycline,sulfonamide,andβ-lactam ARGs were dominant.The high abundance and detection rate of two sulfonamide ARGs(sul1 and sul2)suggested that they were the predominant ARGs.No polymyxin resistance genes(mcr-1)were detected,which indicated that the antibiotic restriction policy of China has achieved positive outcomes.Compared with that in other environmental media,the ARGs abundance in the reservoir environment was low.The correlation analysis showed relevance between the water quality indicators and the ARGs,which suggested that the water quality indexes can be used as ARGs pollution indicators in the reservoir environment.The abundance and detection rate of carbapenem ARGs were low owing to their dosage restriction and high degradability.Tetracycline ARGs were closely related to the other resistance gene types,which might have been due to horizontal gene transfer.Although the overall correlation between intll and ARGs was modest,it might be the main reason for the spread of several individual ARGs in the reservoir environment.

关 键 词：供水水库 抗生素抗性基因 季节变化 影响因素 华中地区 

分 类 号：X524[环境科学与工程—环境工程]