Assessment of CareStart G6PD rapid diagnostic test and CareStart G6PD biosensor in Mauritania  

作　　者：Oum Kelthoum Mamadou Djigo Yacoub Ould Khalef Mohamed Salem Ould Ahmedou Salem Nicolas Gomez Leonardo Basco Sebastien Briolant Ali Ould Mohamed Salem Boukhary 

机构地区：[1]Unite de Recherche"Genomes et Milieux"(Jeune Equipe Associee a I'lnstitut de Recherche pour le Developpement),Faculte des Sciences et lechniques,Universite de Nouakchott Al-Aasriya,Nouakchott,Mauritania [2]Service de Pediatrie,Centre Hospitalier Mere et Enfant,Nouakchott,Mauritania [3]IHU,Mediterrande Infection,Marseille,France [4]Aix Marseille Univ,IRD,AP-HM,SSA,VITROME,Marseille,France [5]Unite de Parasitologie Entomologie,Departement de Microbiologie et Maladies Infectieuses,Institut de Recherche Biomedicale des Armees(IRBA),Marseille,France

出　　处：《Infectious Diseases of Poverty》2021年第4期33-45,共13页贫困所致传染病（英文）

摘　　要：Background::The elimination of Plasmodium vivax malaria requires 8-aminoquinolines,which are contraindicated in patients with glucose-6-phosphate dehydrogenase(G6PD)deficiency due to the risk of acute haemolytic anaemia.Several point-of-care devices have been developed to detect G6PD deficiency.The objective of the present study was to evaluate the performance of two of these devices against G6PD genotypes in Mauritania.Methods::Outpatients were screened for G6PD deficiency using CareStart?rapid diagnostic test(RDT)and CareStart?G6PD biosensor in Nouakchott,Mauritania,in 2019-2020.African-type and Mediterranean-type G6PD genotypes commonly observed in Africa were determined by polymerase chain reaction-restriction fragment length polymorphism and sequencing.Qualitative variables were compared using Fisher’s exact test.Results::Of 323 patients(74 males and 249 females),5 males and 2 homozygous females had the African-type A-genotype:A-(202)in 3 males and 2 females and G6PD A-(968)in 2 males.Among heterozygous females,13 carried G6PD A-(202),12 G6PD A-(968),and 3 G6PD A-(542)variants.None had the Mediterranean-type G6PD genotype.Eight had a positive G6PD RDT result,including all 7 hemizygous males and homozygous females with A-or A-A-(0.12 to 2.34 IU/g haemoglobin,according to G6PD biosensor),but RDT performed poorly(sensitivity,11.1%at the cutoff level of<30%)and yielded many false negative tests.Thirty-seven(50.0%)males and 141(56.6%)females were anaemic.The adjusted median values of G6PD activity were 5.72 and 5.34 IU/g haemoglobin in non-anaemic males(n=35)and non-anaemic males and females(n=130)with normal G6PD genotypes using G6PD biosensor,respectively.Based on the adjusted median of 5.34 IU/g haemoglobin,the performance of G6PD biosensor against genotyping was as follows:at 30%cut-off,the sensitivity and specificity were 85.7%and 91.7%,respectively,and at 80%cut-off,the sensitivity was 100%while the specificity was 64.9%.Conclusions::Although this pilot study supports the utility of biosensor to screen fo

关 键 词：Glucose-6-phosphate dehydrogenase MALARIA Plasmodium vivax PRIMAQUINE Tafenoquine 

分 类 号：R440[医药卫生—诊断学]