关节内注射不同浓度臭氧水对骨性关节炎兔关节软骨的影响  

Effect of intra-articular injection of different concentrations of ozonated water on articular cartilage of rabbits with osteoarthritis

作　　者：肖旭洋 冀恒涛[2] 张真[3] 周友龙[4] 庄志刚[1] 董铁立[1] Xiao Xuyang;Ji Hengtao;Zhang Zhen;Zhou Youlong;Zhuang Zhigang;Dong Tieli(Department of Anesthesiology,The Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450014,China;Department of Laboratory Medicine,The Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450014,China;Department of Anesthesiology,Central Hospital of Zhengzhou University,Zhengzhou 450052,China;Department of Pain Management,Third Affiliated Hospital of Henan University of Traditional Chinese Medicine,Zhengzhou 450004,China)

机构地区：[1]郑州大学第二附属医院麻醉科,450014 [2]郑州大学第二附属医院检验科,450014 [3]郑州大学中心医院麻醉科,450052 [4]河南中医药大学第三附属医院疼痛科,郑州450004

出　　处：《中华麻醉学杂志》2021年第7期844-847,共4页Chinese Journal of Anesthesiology

摘　　要：目的评价关节内注射不同浓度臭氧水对兔骨性关节炎(OA)时关节软骨损伤及软骨细胞凋亡的影响。方法清洁级健康新西兰大白兔24只,雌雄各半,6月龄,体重2.0~3.0 kg,采用随机数字表法分为4组(n=6):对照组(C组)、OA组、低浓度臭氧水组(L组)和高浓度臭氧水组(H组)。采用木瓜蛋白酶法制备OA模型,2周后,L组、H组关节腔分别注射10.0、20.0μg/ml臭氧水1.0 ml,OA组注射0.9%氯化钠溶液1.0 ml,1次/周,共3次。末次注射1周后,取膝关节软骨组织,行甲苯胺蓝染色,光镜下评测Mankin评分;采用ELISA法检测软骨细胞caspase-3活性。结果与C组比较,其余各组Mankin评分、caspase-3活性升高(P<0.05);与OA组比较,L组和H组Mankin评分、caspase-3活性降低(P<0.05);与L组比较,H组Mankin评分升高,caspase-3活性降低(P<0.05)。结论膝关节内注射10.0、20.0μg/ml臭氧水均可抑制软骨细胞凋亡,减轻OA兔关节软骨损伤,而高浓度臭氧水可引起关节软骨基质变性。Objective To evaluate the effect of intra-articular injection of different concentrations of ozonated water on articular cartilage of rabbits with osteoarthritis(OA).Methods Twenty-four clean-grade New Zealand white rabbits of both sexes,weighing 2.0-3.0 kg,aged 6 months,were divided into 4 groups(n=6 each)using a random number table method:control group(group C),OA group,low concentration ozonated water group(L group)and high concentration ozonated water group(H group).The OA model was established by intra-articular injection of papain.At 2 weeks after the model was successfully established,10.0 and 20.0μg/ml ozonated water 1.0 ml was injected into the knee joint of rabbits in L and H groups,respectively,and 0.9%sodium chloride solution 1.0 ml was injected once a week,3 times in total in OA group.At 1 week after the last injection,the cartilage tissue of the knee joint was removed and stained with toluidine blue for evaluation of Mankin score(under light microscope).The activity of caspase-3 in chondrocyte was detected by enzyme-linked immunosorbent assay.Results Compared with group C,the Mankin score and caspase-3 activity were significantly increased in the other 3 groups(P<0.05).Compared with group OA,the Mankin score and caspase-3 activity were significantly decreased in group L and group H(P<0.05).Compared with group L,the Mankin score was significantly increased,and the activity of caspase-3 was decreased in group H(P<0.05).Conclusion Injecting ozonated water 10.0μg/ml and 20.0μg/ml into the knee joint cavity both can inhibit the apoptosis in chondrocytes and reduce the damage to articular cartilage,however,high concentration of ozonated water can cause the denaturation of the articular cartilage matrix in rabbits with OA.

关 键 词：臭氧 注射 关节内 骨关节炎 膝 软骨细胞 软骨 关节 

分 类 号：R684.3[医药卫生—骨科学]