基于JAK3/STAT3信号通路探讨风湿宁对寒湿复合型类风湿关节炎滑膜细胞自噬的影响  

Effects of Fengshining on autophagy of cold-damp composite rheumatoid arthritis synovial cells via JAK3/STAT3 signaling pathway

作　　者：刘涛 王永辉 LIU Tao;WANG Yong-hui(Shanxi University of Chinese Medicine,Jinzhong 030619,China)

机构地区：[1]山西中医药大学,晋中030619

出　　处：《中华中医药杂志》2021年第9期5195-5199,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(No.81473589)。

摘　　要：目的:从JAK3/STAT3信号通路探讨风湿宁对寒湿复合型类风湿关节炎(RA)细胞自噬的影响。方法:制备CIA+寒湿复合型类风湿关节炎大鼠模型,将大鼠按随机数表法分为模型对照组,托法替尼组,风湿宁低、中、高剂量组,另设正常对照组,每组12只,给予蒸馏水或相应药物灌胃进行干预,每日1次,连续28d。ELISA法检测大鼠血清白细胞介素(IL)-2、IL-7含量,PCR法测大鼠滑膜JAK3、STAT3水平,Western blot法测大鼠滑膜Beclin-1、p62水平。结果:与模型对照组比较,各给药组大鼠膝关节滑膜Beclin-1蛋白表达升高(P<0.05),p62蛋白表达下降(P<0.05),风湿宁中剂量组IL-2、IL-7、JAK3 mRNA、STAT3 mRNA表达降低(P<0.05)。结论:风湿宁能降低寒湿复合型RA的炎性因子表达,抑制JAK3/STAT3信号通路活化,进而调控细胞自噬相关蛋白,增强滑膜细胞自噬从而抑制RA滑膜的不断增殖。Objective: To explore the effects of Fengshining(FSN) on autophagy of cold-damp composite rheumatoid arthritis(RA) synovial cells via JAK3/STAT3 signaling pathway. Methods: A rat model of RA of CIA+cold-damp composite model was prepared. The rats were divided into model control group, tofacitinib group, FSN low, medium and high dose groups according to the random number table method. A normal control group was set up with 12 rats in each group. The rats were given intragastric administration with distilled water or corresponding drugs, once a day for 28 consecutive days. The contents of IL-2 and IL-7 in rat serum were detected by ELISA. The levels of JAK3 and STAT3 in the synovium were detected by PCR. Western blot method was used to detect the levels of Beclin-1 and p62 in synovium of rats. Results: Compared with the model control group, Beclin-1 protein expression increased(P<0.05) and p62 protein expression decreased(P<0.05) in the synovium of the knee joint of the rats in each treatment group. The expressions of IL-2, IL-7, JAK3 mRNA, and STAT3 mRNA were decreased in the middle-dose group(P<0.05). Conclusion: FSN reduces the expression of inflammatory factors of cold-damp composite RA,inhibits the activation of JAK3/STAT3 signaling pathway, regulates autophagy related proteins and enhances the autophagy of synovial cells, thus inhibiting the continuous proliferation of RA synovium.

关 键 词：风湿宁 类风湿关节炎 JAK3/STAT3信号通路 自噬 

分 类 号：R285[医药卫生—中药学]