黄芪多糖对低氧环境下大鼠膀胱平滑肌细胞缝隙连接蛋白43表达的影响  

Effects of astragalus polysaccharides on the expression of Connexin43 in the rat bladder smooth muscle cells under hypoxic condition

作　　者：赵凡 叶妙勇 张春和 ZHAO Fan;YE Miao-yong;ZHANG Chun-he(Affiliated Hospital of Nantong University,Nantong 226001,China;The Affiliated Wenling Hospital of Wenzhou Medical University,Wenling 317500,China;Yunnan Provincial Hospital of Traditional Chinese Medicine/The First Affiliated Hospital of Yunnan University of Chinese Medicine,Kunming 650021,China)

机构地区：[1]南通大学附属医院,南通226001 [2]温州医科大学附属温岭医院,浙江温岭317500 [3]云南省中医医院/云南中医药大学第一附属医院男科,昆明650021

出　　处：《中华中医药杂志》2021年第9期5577-5580,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81860853,No.81774323,No.82004360)。

摘　　要：目的:探索黄芪多糖对低氧环境下SD大鼠膀胱平滑肌细胞缝隙连接蛋白43(CX43)表达的影响。方法:酶消化法分离SD大鼠原代膀胱平滑肌细胞后进行培养、鉴定;构建体外膀胱平滑肌细胞缺氧模型,免疫印迹技术检测不同缺氧时间对膀胱平滑肌细胞中缺氧诱导因子-1α(HIF-1α)和CX43表达的影响;用低、高浓度黄芪多糖处理低氧环境下膀胱平滑肌细胞,免疫荧光和免疫印迹技术检测各组膀胱平滑肌细胞中HIF-1α和CX43表达强度。结果:SD大鼠膀胱平滑肌细胞SM-α-actin呈阳性染色;与常氧组比较,低氧环境下培养6、12、24h,膀胱平滑肌细胞中HIF-1α和CX43表达均升高(P<0.05,P<0.01);低氧环境下培养膀胱平滑肌细胞24h的同时予黄芪多糖100μg/mL或400μg/mL进行处理,与低氧组比较,黄芪多糖干预组HIF-1α和CX43表达均受到抑制(P<0.05,P<0.01)。结论:黄芪多糖对低氧环境下大鼠膀胱平滑肌细胞中HIF-1α和CX43蛋白表达升高可起到抑制作用,其中机制尚待进一步研究。Objective: To investigate the effects of astragalus polysaccharides(APS) on the expression of Connexin43(CX43) in the SD rat bladder smooth muscle cells(BSMCs) under hypoxic condition. Methods: Primary BSMCs enzymaticallydigested from the bladder of SD rats were cultivated and identified. Influence of various-interval hypoxic exposure on the expressions of hypoxia-induced factor-1 alpha(HIF-1α) and CX43 in BSMCs were detected by Western blot. Additionally,BSMCs under hypoxic condition were treated by low-or high-dosage of APS after which the expressions of HIF-1α and CX43 in BSMCs were detected by Western blot and Immunocytochemistry. Results: SD rats BSMCs were positively stained by SM-α-actin. Compared with the normoxic group, the protein expressions of HIF-1α and CX43 in BSMCs were increased in 6, 12,24 h(P<0.05, P<0.01). After BSMCs were treated by 100μg/mL or 400μg/mL APS along with hypoxic stimulation for 24 h, the protein expressions of HIF-1α and CX43 in BSMCs which received APS treatment were inhibited when compared to the hypoxic group(P<0.05, P<0.01). Conclusion: The uptrend of HIF-1α and CX43 protein expressions in BSMCs under hypoxia can be alleviated through APS treatment, but the mechanisms in which need a further study.

关 键 词：黄芪多糖 缺氧 膀胱平滑肌细胞 缝隙连接蛋白43 膀胱出口梗阻 

分 类 号：R285.5[医药卫生—中药学]