检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:刘必旺 赵换 马一方 张惠忠 燕小宁 栾智华 马艳苗 周文静 封雪 赵俊云[4] 杨向竹[4] Baukje de Roos LIU Bi-wang;ZHAO Huan;MA Yi-fang;ZHANG Hui-zhong;YAN Xiao-ning;LUAN Zhi-hua;MA Yan-miao;ZHOU Wen-jing;FENG Xue;ZHAO Jun-yun;YANG Xiang-zhu;Baukje de Roos(Shanxi University of Chinese Medicine,Jinzhong 030619,China;Affiliated Hospital of Shanxi University of Chinese Medicine,Taiyuan 030024,China;Shanxi Environmental Monitoring Center,Taiyuan 030027,China;Beijing University of Chinese Medicine,Beijing 100029,China;University of Aberdeen.Aberdeen AB243FX,UK)
机构地区:[1]山西中医药大学实验基本技术教研室,晋中030619 [2]山西中医药大学附属医院,太原030024 [3]山西省环境监测中心,太原030027 [4]北京中医药大学,北京100029 [5]英国阿伯丁大学,阿伯丁AB243FX [6]山西中医药大学,晋中030619
出 处:《中华中医药杂志》2021年第9期5581-5585,共5页China Journal of Traditional Chinese Medicine and Pharmacy
基 金:国家自然科学基金项目(No.81102553);山西省国际合作重点项目(No.201603D4210108);山西省卫生计生委员会项目(No.201601102);山西中医药大学中医方剂新药创制科技创新团队项目(No.2018TD-004)。
摘 要:目的:探讨黄芪发酵产物拮抗PM2.5肺损伤的干预效果,为黄芪研究及其发酵产物的开发利用提供参考依据。方法:通过电感耦合等离子体发射光谱仪确定PM2.5重金属的主要成分。将36只Wistar大鼠随机分成空白对照组,染毒组,黄芪组,黄芪发酵产物低、中、高剂量组,根据文献采用气管滴注方法复制PM2.5肺损伤的动物模型,各组相应处理4周,最后1次滴注后,进行微循环动态可视化观察研究,并进行血气分析检测。录制直径在30~50μm的肺细静脉白细胞黏附动态视频,计算200μm长的肺细静脉内粘附白细胞数量。结果:本研究PM2.5的重金属成分以铁(Fe)、锌(Zn)、铬(Cr)为主。与空白对照组比较,染毒组肺组织白细胞聚集严重(P<0.01),PCO_(2)显著升高(P<0.05),PO_(2)和SO_(2)显著降低(P<0.05)。与染毒组比较,黄芪组及黄芪发酵产物中、高剂量组黏附于肺细静脉血管壁的白细胞数量显著减少(P<0.05),PCO_(2)显著降低(P<0.05),PO_(2)和SO_(2)显著升高(P<0.05)。结论:黄芪发酵产物能使PM2.5损伤的肺组织炎性程度降低,肺功能得到改善,对临床上呼吸系统疾病防治及其黄芪科学研究具有一定的启示作用。Objective: To explore the intervention effect of Astragali Radix fermentation products on lung injury caused by PM2.5, and provide a reference for the research of Astragali Radix and the development and utilization of its fermentation products. Methods: The main components of PM2.5 heavy metals were determined through inductively coupled plasma emission spectrometer. Then, 36 Wistar rats were randomly divided into NC group, MC group, HQ group, HFL group, HFM group,HFH group. According to the literature, the animal model of PM2.5 lung injury was reproduced by tracheal instillation. Each group was treated for 4 weeks. Micro-circulation dynamic visual observation was conducted after the last instillation, and the blood gas analysis was performed. This study observed the pulmonary vein with a diameter of 30~50μm, recorded the dynamic video of leukocyte adhesion, and calculated the number of leukocyte adhesion in the pulmonary vein with a length of 200μm.Results: In this study, the heavy metal components of PM2.5 were mainly Fe, Zn and Cr. Compared with NC group, in the MC group leukocyte aggregation was serious in lung tissue(P<0.01), PCO_(2) increased significantly(P<0.05), PO_(2) and SO_(2) decreased significantly(P<0.05). Compared with MC group, in HQ group, HFM group and HF group the leukocytes adhering to the pulmonary vein wall decreased significantly(P<0.01), PCO_(2) decreased significantly(P<0.05), PO_(2) and SO_(2) increased significantly(P<0.05). Conclusion: Astragali Radix and its fermentation products can reduce the inflammatory degree of lung tissue damaged by PM2.5 and improve the lung function, which has a certain enlightenment on the prevention and treatment of respiratory diseases and the scientific research of Astragali Radix.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.38