3D打印技术制备β-磷酸三钙仿生骨支架在兔股骨髁部骨缺损修复中的应用  

Repair of femoral condyle bone defect in rabbits withβ-tricalcium phosphate bionic stent prepared by 3D printing technology

作　　者：陈宏亮 郭开今 陈向阳 段钢 Chen Hongliang;Guo Kaijin;Chen Xiangyang;Duan Gang(Department of Orthopaedics,Affiliated Hospital of Xuzhou Medical University,Xuzhou 221000,China;Department of Orthopaedics,the Second Affiliated Hospital of Xuzhou Medical University,Xuzhou 221000,China)

机构地区：[1]徐州医科大学附属医院骨科,徐州221000 [2]徐州医科大学第二附属医院骨科,徐州221000

出　　处：《中华解剖与临床杂志》2021年第5期571-577,共7页Chinese Journal of Anatomy and Clinics

基　　金：江苏省科技厅自然科学基金面上项目(BK20201154)。

摘　　要：目的探讨采用3D打印技术制备的β-磷酸三钙(β-TCP)仿生骨支架的形态结构特点及其相关生物性能,并观察其修复新西兰兔股骨髁部骨缺损的效果。方法选取5~6月龄新西兰大白兔20只,随机分为支架组和空白组,每组10只;两组大白兔按造模术后采集标本的时间不同又分为两个亚组,每组5只。两组大白兔均于左侧股骨用环钻钻取直径约5 mm、长约10 mm的圆柱形松质骨块,建立股骨髁骨缺损模型。空白组截取的10个松质骨标本,使用微计算机断层扫描技术进行扫描,获得骨缺损标本的结构影像学数据,通过3D生物打印系统设计出相应的仿生骨支架模型,再以β-TCP作为打印材料,打印出20枚仿生骨支架。取10枚β-TCP支架测量高度、直径,电子显微镜下观察β-TCP支架孔道形态结构特点,测量大孔的直径和孔隙率,使用电子力学测试机测定β-TCP支架的弹性模量与抗压强度。空白组10只大白兔造模后不植入任何材料。支架组10只大白兔在造模后,将制备的10枚β-TCP支架植入骨缺损处。分别于术后第6、12周使用耳缘静脉推注空气方法处死空白组和支架组的各亚组大白兔,于骨缺损部位或植骨部位上下离断、截取长约10 mm骨段,制备切片,HE染色,观察骨组织生长情况;采用Lane-Sandhu组织学评分标准对骨组织修复情况进行评价。结果使用3D生物打印技术制备的20枚圆柱体β-TCP支架,与松质骨标本结构形态相似。支架高度(9.97±0.08)mm、直径(5.09±0.07)mm,松质骨标本高度(9.96±0.39)mm、直径(5.01±0.22)mm,支架与松质骨标本比较差异均无统计学意义(P值均>0.05)。扫描电镜观察到支架表面及内部呈均匀多孔状,孔径相互连通,大小相仿,孔隙分布较均匀,在大孔侧壁布满了微孔,外形多为近似圆形;其中大孔直径为(223.02±18.20)μm,孔隙率为74.02%±1.38%。松质骨标本大孔直径(227.02±31.20)μm,孔隙率为76.02%±3.29%,支架与松Objective To analyze the morphological and structural characteristics and related biological properties ofβ-tricalcium phosphate(β-TCP)biomimetic bone scaffold prepared by 3D printing technology and evaluate the effectiveness of this bone scaffold in repairing femoral condyle bone defect in New Zealand rabbits.Methods Twenty white New Zealand rabbits aged 5 to 6 months were randomly divided into two groups:a stent group and a blank group,with 10 rabbits in each group.According to the time of specimen collection after modeling,these groups were further divided into two subgroups with 5 rabbits in each group.A cylindrical cancellous bone block with a diameter of 5 mm and a length of 10 mm was drilled from the left femur of the two groups of white rabbits to establish the model of femoral condyle bone defect.Ten interceptor cancellous bone specimens were obtained from the blank group.The structure of the specimens with bone defect was examined via microcomputer tomography scanning.The corresponding bionic bone scaffold model was designed and fabricated via 3D printing.Withβ-TCP as the printing material,20 bionic bone scaffolds were printed.The height and diameter of the 10β-TCP stents were measured.The morphology and structure of theβ-TCP scaffolds were observed under an electron microscope,and the diameter and porosity of macropores were measured.The elastic modulus and compressive strength ofβ-TCP brackets were measured using an electronic mechanical tester.The 10 white rabbits in the blank group were not implanted with any material after modeling.After the femoral condyle defects were modeled,the 10β-TCP scaffolds were implanted into the bone defects of the 10 white rabbits in the scaffold group.At 6 and 12 weeks after the operation,the rabbits in the two groups were sacrificed by auricular vein injection.Bone segments with a length of about 10 mm were cut off above and below the bone defect site or the bone graft site.The sections were prepared and stained with Hematoxylin-eosin to observe the growth of b

关 键 词：组织支架 Β-磷酸三钙 三维打印技术 动物实验 骨缺损 

分 类 号：R318.08[医药卫生—生物医学工程]