高效液相色谱法同时测定姜天麻饮片中天麻素和对羟基苯甲醇含量  

Simultaneous Determination of Gastrodin and p-Hydroxbenzyl Alcohol in Jiangtianma by HPLC

作　　者：唐庆 杨德泉 佘永红 TANG Qing;YANG Dequan;SHE Yonghong(Xiangxi Tujia and Miao Autonomous Prefecture of Food and Drug Inspection Office,Jishou Hunan 416000,China)

机构地区：[1]湘西土家族苗族自治州食品药品检验所,湖南吉首416000

出　　处：《药品评价》2021年第17期1046-1049,共4页Drug Evaluation

基　　金：湖南省自然科学基金项目(2021JJ80030)。

摘　　要：目的:建立姜天麻饮片中天麻素和对羟基苯甲醇的含量测定方法。方法:采用高效液相色谱法对姜天麻中天麻素和对羟基苯甲醇含量进行测定。色谱柱为YMC-C18(250 mm×4.6 mm,5μm),流动相乙腈-0.05%磷酸溶液(3∶97),流速0.8 mL/min;检测波长为220 nm;柱温35℃。理论板数按天麻素峰计算,不低于5000,研究测定姜天麻饮片的天麻素和对羟基苯甲醇含量。结果:天麻素和对羟基苯甲醇分别在0.9521~152.3416μg/mL和0.4493~71.8860μg/mL范围内线性关系良好(r均为0.9999),天麻素及对羟基苯甲醇平均加样回收率分别为99.18%、95.21%,RSD分别为2.65%(n=9)、2.75%(n=9)。结论:HPLC含量测定方法简单、快速、准确,可用于姜天麻饮片的质量控制。Objective:To establish a method for simuitaneous determination of gastrodin and p-hydroxbenzyl alcohol in Jiangtianma.Methods:The contents of gastrodin and p-hydroxbenzyl alcoholin Jiangtianma were determined by HPLC.The chromatographic column was YMC HPLC Column C18(250 mm×4.6 mm,5μm).The mobile phase was acetonitrile-0.05%phosphoric acid solution(3∶97).The flow rate was 0.8 mL/min.The detection wavelength was 220 nm and the column temperature set at 35℃.The theoretical plate number was calculated according to gastrodin peak,not less than 5000,the contents of gastrodin and p-hydroxbenzyl alcohol in Jiangtianma were determined.Results:The linear ranges of gastrodin and p-hydroxbenzyl alcohol were 0.9521-152.3416μg/mL(r=0.9999)and 0.4493-71.8860μg/mL,(r=0.9999)respectively.The average recoveries of gastrodin and p-hydroxbenzyl alcohol were 99.18%(RSD=2.65%,n=9)and 95.21%(RSD=2.75%,n=9)respectively.Conclusion:The HPLC method is simple,rapid and accurate,and can be used for the quality control of Jiangtianma.

关 键 词：天麻属 主成分分析 姜天麻 天麻素 对羟基苯甲醇 高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学]