土壤酸化对红枫叶片呈色生理的影响  

Effects of Soil Acidification on Leaf Color Physiology of Acer palmatum ’Atropurpureum’

作　　者：靳慧琴 梁俊林 贾诗雨 肖欢 唐实玉 冯云超 刘洋 JIN Huiqin;LIANG Junlin;JIA Shiyu;XIAO Huan;TANG Shiyu;FENG Yunchao;LIU Yang(Institute of Ecology&Forestry,Sichuan Agricultural University/Key laboratory of Ecological Forestry Engineering of Sichuan Province,Chengdu 611130,China;The College of Forestry of Beijing Forestry University,Beijing 100083,China;SichuanWest Forestry Bureau of Aba Tibetan and Qiang Autonomous Prefecture in Sichuan Province,Lixian 623102,Sichuan,China)

机构地区：[1]四川农业大学林学院生态林业研究所/长江上游林业生态工程四川省重点实验室,成都611130 [2]北京林业大学林学院,北京100083 [3]四川省阿坝藏族羌族自治州理县林业和草原局,四川理县623102

出　　处：《四川农业大学学报》2021年第5期590-595,625,共7页Journal of Sichuan Agricultural University

基　　金：省级大学生创新创业训练计划项目(201910626063);四川省中央引导地方科技发展专项(2020ZYD049);四川省重点研发计划项目(18ZDYF0307)。

摘　　要：【目的】研究土壤酸化对红枫秋季叶片呈色生理的影响,为亚高山彩叶林红枫的引种栽培提供科学依据。【方法】于2018年8月—2019年11月使用pH6和pH4柠檬酸缓冲液对土壤进行酸化处理,分析两个年份红枫叶片转色期的色素含量(叶绿素、类胡萝卜素、花色素苷),酶活性(叶绿素酶、苯丙氨酸解氨酶)及内含物质(脯氨酸、可溶性糖)等生理指标。【结果】红枫叶色转变过程中,叶片叶绿素、类胡萝卜素和可溶性糖含量降低,花色素苷及脯氨酸含量增加,叶绿素酶和苯丙氨酸解氨酶活性没有显著变化。在不同处理间,pH4柠檬酸缓冲液处理延缓了叶绿素和类胡萝卜素的降解且各时期花色素苷的积累量最低,而pH6柠檬酸缓冲液处理在2019年加速了叶绿素的降解且花色素苷含量显著高于对照;pH6和pH4酸化处理的叶绿素酶活性在2018年高于对照,而苯丙氨酸解氨酶活性在2019年高于对照;各采样时期pH6柠檬酸缓冲液处理的脯氨酸和可溶性糖含量均高于对照。【结论】使用pH6柠檬酸缓冲液酸化处理有利于红枫转色期叶绿素的降解及花色素苷的合成。因此,使用pH6柠檬酸缓冲液适当降低土壤碱性可使红枫提前转色及增强呈色效果,而pH4柠檬酸缓冲液处理的叶色转变效果不佳。【Objective】The effects of soil acidification on leaf color physiology of Acer palmatum‘Atropurpureum’in autumn were studied to provide scientific basis for the introduction and cultivation of Acer palmatum‘Atropurpureum’in subalpine colored leaf forest.【Method】From August 2018 to November 2019,pH6 and p H4 citric acid buffers were used to acidify the soil,and the pigment content(chlorophyll,carotenoid,anthocyanin),enzyme activity(chlorophyllase,phenylalanine ammonialyase),contained substances(proline,soluble sugar) and other physiological indicators of Acer palmatum‘Atropurpureum’leaves during the color change period of the two years was analyzed.【Result】During the leaf color transformation of Acer palmatum‘Atropurpureum’,the contents of chlorophyll,carotenoid and soluble sugar decreased,the contents of anthocyanin and proline increased,and the activities of chlorophyllase and phenylalanine ammonia lyase did not change significantly. Among different treatments,the pH4 citric acid buffer treatment delayed the degradation of chlorophyll and carotenoids and the accumulation of anthocyanins was the lowest in each period,while the pH6 citrate acid buffer treatment in 2019 accelerated the degradation of chlorophyll and the anthocyanin content was significantly higher than that of the control;The chlorophyllase activity of pH6 and pH4 acidification treatment was higher than the control in2018,and the activity of phenylalanine ammonia lyase was higher than the control in 2019;The proline and soluble sugar content of pH6 citric acid buffer treatment in each sampling period were higher than the control.【Conclusion】The acidification treatment with pH 6 citric acid buffer is beneficial to the degradation of chlorophyll and the synthesis of anthocyanins during the color conversion period of Acer palmatum‘Atropurpureum’. Therefore,the use of pH 6 citric acid buffer to appropriately reduce soil alkalinity can make Acer palmatum‘Atropurpureum’color change in advance and enhance the color r

关 键 词：红枫 色素 叶片呈色 土壤酸化 

分 类 号：S792.35[农业科学—林木遗传育种]