无患子叶斑病病原菌鉴定及其生物学特性  

Identification and Biological Characteristics of Pathogen Causing Leaf Spot Disease of Sapindus mukorossi

作　　者：郑婉铮 孙宇 林先云 陈全助[3] 冯锦秀 蔡琦 冯丽贞[1] ZHENG Wanzheng;SUN Yu;LIN Xianyun;CHEN Quanzhu;FENG Jinxiu;CAI Qi;FENG Lizhen(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Shunchang County Forestry Bureau,Shunchang 353200,Fujian,China;Jinshan College,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学林学院,福州350002 [2]顺昌县林业局,福建顺昌353200 [3]福建农林大学金山学院,福州350002

出　　处：《四川农业大学学报》2021年第5期613-619,共7页Journal of Sichuan Agricultural University

基　　金：福建省财政厅资助项目(K8113001A)。

摘　　要：【目的】明确福建顺昌无患子叶斑病病原及其生物学特性。【方法】以福建省顺昌县受害无患子人工林基地采集的病样组织为研究对象,通过组织分离法、致病性测定、形态学鉴定及分子生物学鉴定,确定无患子叶斑病病原菌种类,在此基础上探究病原菌生物学特性。【结果】通过组织分离法从发病部位分离出9种菌株,经柯赫氏法则验证,确定SW-9为无患子叶斑病病原菌。结合传统形态学和分子生物学,将该菌鉴定为Xylaria arbuscula(矮乔木炭角菌)。SW-9菌株最适生长温度为28℃;最适生长pH为6~7;最适碳源为麦芽糖,最适氮源为蛋白胨;其致死温度为40℃;最适光照条件为全黑暗培养。【结论】关于Xylaria arbuscula引起的无患子叶斑病为首次报道,研究结果将为开展无患子叶斑病的防治工作奠定基础。【Objective】To clarify the pathogen causing leaf spots of Sapindus mukorossi in Shunchang County and its biological characteristics.【Method】Taking the disease sample tissue collected from the Sapindus mukorossi artificial forest base in Shunchang County,Fujian Province as the research object,to determine the types of pathogen by tissue separation,pathogenic determination,morphological identification and molecular biological identification,and explore the biological characteristics of the pathogen.【Result】Nine species of fungi were isolated from the site by tissue separation,SW-9 was identified as pathogen of leaf spot disease of Sapindus mukorossi. Combining traditional morphology and molecular biology,the fungus was identified with Xylaria arbuscula. The most suitable growth temperature is 28 ℃;most suitable growth pH is 6-7;most suitable carbon source is maltose,and most suitable nitrogen source is protein;the death temperature is 40 ℃;most suitable light conditions are all dark culture.【Conclusion】The leaf spot disease of Sapindus mukorossi caused by Xylaria arbuscula is the first reported,and the results will lay the foundation for the prevention and treatment of the leaf spot disease of Sapindus mukorossi.

关 键 词：无患子 叶斑病 病原菌 生物学特性 

分 类 号：S432.1[农业科学—植物病理学]