不同NFC/NDF饲粮对青海藏羊育成母羊肌肉抗氧化功能、肌纤维类型组成及其相关基因表达的影响  

Effects of Different NFC/NDF Diets on Antioxidant Function,Myofiber Type Composition and Related Gene Expression of Qinghai Tibetan Ewes

作　　者：周力 高占红 张春梅 马博妍 李蒋伟 桂林生 侯生珍[1] ZHOU Li;GAO Zhanhong;ZHANG Chunmei;MA Boyan;LI Jiangwei;GUI Linsheng;HOU Shengzhen(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学农牧学院,西宁810016

出　　处：《四川农业大学学报》2021年第5期639-645,共7页Journal of Sichuan Agricultural University

基　　金：青海省林草局项目(青海湖-祁连山保护体系,K992138);青海省农牧业科技创新平台项目(QNKP20180602);青海省科技厅项目(藏羊高效养殖技术集成与示范推广,091721301064071017);青海大学校地合作项目(黑藏羊生长发育关键基因挖掘及开发研究,2020GN118)。

摘　　要：【目的】研究不同非纤维性碳水化合物(NFC)/中性洗涤纤维(NDF)饲粮对育成期藏羊(♀)背最长肌的肌纤维特性、肌球蛋白重链基因表达量及其抗氧化能力的影响。【方法】选用体况良好、体重(26.65±0.72)kg相近的4月龄青海藏羊母羔15只,随机分为3组,进行预试期15 d和正试期90 d的饲养试验。正试期3个组饲粮NFC/NDF水平分别为0.92(L组)、1.39(M组)、2.20(H组)。以藏羊左侧胴体第12~13肋骨间背最长肌作为试验材料,利用酶联免疫吸附法(ELISA)、三磷酸腺苷酶(ATPase)染色和实时荧光定量PCR(real-time qPCR)方法进行测定。【结果】①M组Ⅰ型肌纤维直径小于H组(P<0.05)与L组(P>0.05);M组Ⅰ型肌纤维横截面积低于L组(P<0.05)与H组(P>0.05);M组Ⅰ型肌纤维密度高于H组(P<0.05)和L组(P>0.05);L组Ⅰ型肌纤维数量和面积显著小于M组(P<0.05),而Ⅱb型肌纤维数量和面积则相反(P<0.05)。②M组MyHCⅠ基因的表达量显著高于L组(P<0.05),而L组MyHCⅡa基因的表达量显著高于H组(P<0.05),同时L组MyHCⅡb和MyHCⅡx的基因的表达量显著高于M组(P<0.05)。③M组过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性大于L组(P<0.05)与H组(P>0.05);M组谷胱甘肽过氧化物酶(GSH-Px)活性大于H组(P<0.05)与L组(P>0.05)。【结论】不同NFC/NDF饲粮能够影响育成藏母羊肌肉抗氧化能力、肌纤维类型组成及其相关基因的表达量,其中以NFC/NDF为1.39的饲粮效果较佳。【Objective】This experiment was conducted to study the effects of different non fibrous carbohydrate(NFC)/neutral detergent fiber(NDF)diets on the characteristics of muscle fiber,myosin heavy chains(MyHCs)genes expression,and antioxidant capacity of longissimus dorsi muscle in growing Tibetan sheep(♀).【Method】Fifteen 4-months-old female lambs of Qinghai Tibetan sheep with similar body weight of(26.65±0.72)kg were selected and randomly divided into three groups.The pre-experiment period lasted for 10 days,and the experiential period lasted for 90 days.The NFC/NDF levels of the three groups were 0.92,1.39,and 2.20,named group L,M,and H,respectively.The longissimus dorsi muscle between the left 12th and 13th ribs of the carcass was used as the test material.Enzyme linked immunosorbent assay(ELISA),adenosine triphosphatase(ATPase)staining and real-time PCR were used to determine the content.【Result】The results showed that:①TheⅠmuscle fiber diameter of group M was smaller than that in group H(P<0.05)and L(P>0.05);The cross-sectional area of muscle fiber typeⅠin group M was lower than that in group L(P<0.05)and group H(P>0.05).The muscle fiber density of M group was higher than that in H group(P<0.05)and L group(P>0.05).The number and area of typeⅠmuscle fiber in Group L were significantly lower than those in group M(P<0.05),while the number and area of typeⅡb muscle fiber were opposite(P<0.05).②The expression level of MyHCⅠgene in group Mwas significantly higher than that in group L(P<0.05),while the expression level of MyHCⅡa gene in group L was significantly higher than that in group H(P<0.05).and the expression level of MyHCⅡb and MyHCⅡx genes in group L was significantly higher than that in group M(P<0.05).③The activities of catalase(CAT)and superoxide dismutase(SOD)in group M were higher than those in group L(P<0.05)and group H(P>0.05).The activity of glutathione peroxidase(GSH-Px)in group M was higher than that in group H(P<0.05)and group L(P>0.05).【Conclusion】Therefore,di

关 键 词：高原型藏羊 精粗比 非纤维性碳水化合物/中性洗涤纤维 抗氧化能力 MyHCs基因 

分 类 号：S858.26[农业科学—临床兽医学]