肌肉RAS癌基因多态性与高血压及高血压合并缺血性脑卒中的关系  

Association between muscle RAS oncogene homolog polymorphism and hypertension and hypertension with ischemic stroke

作　　者：方正美 朱丽君 宋岩 陈燕 张晓宇 朱连见 金岳龙 常微微 姚应水 FANG Zheng-mei;ZHU Li-jun;SONG Yan;CHEN Yan;ZHANG Xiao-yu;ZHU Lian-jian;JIN Yue-long;CHANG Wei-wei;YAO Ying-shui(School of Public Health/Institute of Chronic Disease Control and Prevention,Wannan Medical College,Wuhu 241002,Anhui Province,China;Department of Medical Imaging,Jieshou People's Hospital,Fuyang 236000,Anhui Province,China;Department of Clinical Laboratory,Jieshou People's Hospital,Fuyang 236000,Anhui Province,China;Department of Clinical Nutrition,Hefei BOE Hospital,Hefei 230000,Anhui Province,China;Department of Medicine,Anhui College of Traditional Chinese Medicine,Wuhu 241002,Anhui Province,China)

机构地区：[1]皖南医学院公共卫生学院/慢性病防制研究所,安徽芜湖241002 [2]界首市人民医院影像科,安徽阜阳236000 [3]界首市人民医院检验科,安徽阜阳236000 [4]合肥京东方医院临床营养科,安徽合肥230000 [5]安徽中医药高等专科学校医学系,安徽芜湖241002

出　　处：《中国临床药理学杂志》2021年第19期2553-2557,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81673266,81874280);安徽省教育厅高校自然科学研究基金资助项目(KJ2019A0404,KJ2019A0405)。

摘　　要：目的探讨肌肉RAS癌基因(MRAS)位点rs40593、rs751357和rs6782181多态性与高血压(EH)、EH合并缺血性脑卒中(EH+IS)之间的关系,并进一步分析MRAS与常用降压药疗效的关系。方法根据血压以及是否合并IS将其分为健康对照组(410人),单纯EH组(282人)和EH+IS组(301人)。用Taq Man探针法对3个位点(rs40593、rs751357和rs6782181)进行基因分型。用卡方检验和多分类Logistic回归分析对MRAS多态性与EH、EH+IS的关系进行统计分析。结果在总人群中,未发现3个位点与EH、EH+IS的关联,不同基因型间服用降压药后血压水平差异均无统计学意义。进一步对性别进行分层分析发现,在女性人群中,rs40593的突变等位基因在对照组、单纯EH组和EH+IS组的分布频率分别为22.33%,32.71%和25.16%,rs751357的突变等位基因分布频率分别为23.54%,34.33%和26.45%,rs6782181的突变等位基因分布频率分别为24.03%,33.21%和26.30%,EH组的上述指标与对照组比较,差异均有统计学意义(均P <0.05)。多分类Logistic回归分析发现,与对照组相比,MRAS rs40593 GG基因型与女性人群EH存在关联[比值比(OR)=3.10,95%置信区间=1.33~7.23]。结论在女性中,携带MRAS rs40593 GG基因型可能是EH的危险因素。Objective To investigate the relationship between the polymorphism of muscle RAS oncogene homolog(MRAS) rs40593,rs751357 and rs6782181 with essential hypertension(EH) and hypertension complicated with ischemic stroke(EH+IS),and further analyze the relationship between MRAS and the efficacy of commonly used antihypertensive drugs.Methods The subjects were divided into healthy control group(410),EH group(282) and EH+IS group(301) according to their blood pressure and whether they were combined with IS.Three loci(rs40593,rs751357 and rs6782181) were genotyped by TaqMan probe.Chi square test and Logistic regression analysis were used to analyze the relationship between MRAS polymorphism and EH and EH with IS.Results No association was found between the three loci and EH and EH+IS in the total population.The difference in blood pressure levels between different genotypes after taking antihypertensive drugs had no statistical significance.A further stratified analysis of gender found that in female population,the mutant alleles distribution frequencies of the rs40593 in control,EH and EH+IS groups were 22.33%,32.71% and 25.16%,the mutant alleles distribution frequencies of the rs751357 were 23.54%,34.33% and 26.45%,the mutant alleles distribution frequencies of the rs6782181 were 24.03%,33.21% and 26.30%,and compared between control and EH groups,the differences were statistically significant(all P<0.05).Multiple Logistic regression analysis showed that MRAS rs40593 GG genotype was associated with hypertension in female population [odds ratio(OR)=3.10,95% confidence interval=1.33-7.23].Conclusion MRAS rs40593 GG genotype may be a risk factor for hypertension in women.

关 键 词：原发性高血压 缺血性脑卒中 肌肉RAS癌基因 基因多态性 

分 类 号：R97[医药卫生—药品]