野鸢尾黄素对人脑胶质瘤细胞LN229增殖、迁移和侵袭的影响  

Effects of irigenin on the proliferation,migration and invasion of human glioma cell line LN229

作　　者：周扬[1] 朱冰影 周希禛 朱缨[1] 沈敏[1] 钟其新 曾峰 ZHOU Yang;ZHU Bing-ying;ZHOU Xi-zhen;ZHU Ying;SHEN Min;ZHONG Qi-xin;ZENG Feng(Suzhou Key Laboratory of Medical Biotechnology,Pharmacy Department,Suzhou Vocational Health College,Suzhou 215009,Jiangsu Province,China;Oncology Department of Traditional Chinese Medicine,Hangzhou First People's Hospital Affiliated to Zhejiang University Medical College,Hangzhou 310006,Zhejiang Province,China;Department of Geriatrics,Shenzhen Hospital,Guangzhou University of Chinese Medicine,Shenzhen 518034,Guangdong Province,China;Artemisinin Research Center,Guangzhou University of Chinese Medicine,Guangzhou 510445,Guangdong Province,China;Scientific Research Office,The First Affiliated Hospital,Guangzhou University of Chinese Medicine/The First Clinical Medical School,Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China)

机构地区：[1]苏州卫生职业技术学院药学院,苏州检验医学生物技术重点实验室,江苏苏州215009 [2]浙江大学医学院附属杭州市第一人民医院中医肿瘤科,浙江杭州310006 [3]广州中医药大学深圳医院老年病科,广东深圳518034 [4]广州中医药大学青蒿研究中心,广东广州510445 [5]广州中医药大学第一附属医院、广州中医药大学第一临床医学院科研处,广东广州510405

出　　处：《中国临床药理学杂志》2021年第19期2618-2621,共4页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金青年基金资助项目(31901002);江苏省卫生健康委医学科研基金资助项目(Z2019051);苏州市科技局民生科技基金资助项目(SYSD2018072);福田区卫生公益科研基金资助项目(FTWS2019024;FTWS2020050);苏州卫生职业技术学院青年科技专项基金资助项目(SZWZY201711)。

摘　　要：目的观察野鸢尾黄素对人脑胶质瘤LN229细胞的增殖、迁移和侵袭能力,以及基质金属蛋白酶-2(MMP-2)表达的影响。方法将细胞分为3组,分别为空白对照组和低、高剂量实验组。空白对照组不加任何药物;低、高剂量实验组分别加入40和80μmol·L-1野鸢尾黄素。用免疫荧光实验检测细胞增殖能力,用Tranwell小室实验检测细胞的迁移和侵袭能力,用蛋白质印迹法检测MMP-2蛋白的表达水平。结果干预48 h后,空白对照组和低、高剂量实验组的细胞增殖率分别为(38.07±2.53)%,(23.90±1.10)%和(12.00±2.65)%,迁移细胞数量分别为(683.33±110.15),(322.00±72.19)和(132.33±18.23)个,侵袭细胞数量分别为(715.33±109.70),(300.00±60.67)和(135.00±30.64)个,MMP-2蛋白相对表达量分别为0.95±0.05,0.77±0.06和0.27±0.06。低、高剂量实验组的上述指标与空白对照组相比,差异均有统计学意义(P <0.05或P <0.01或P <0.001)。结论野鸢尾黄素可能通过抑制MMP-2的表达,从而抑制LN229细胞的增殖、迁移及侵袭。Objective To observe the effects of irigenin on the viability,proliferation,migration and invasion of human glioma LN229 cells and the expression of matrix metalloproteinase-2(MMP-2).Methods LN229 cells were divided into three groups:blank control group,experimental-L and-H groups.The blank control group did not add any drugs,while the experimental-L and-H groups were added with40 and 80 μmol·L-1 irigenin,respectively.Immunofluorescence assay was used to detect cell proliferation,transwell chamber assay was used to detect cell migration and invasion,and Western Blot was used to detect the expression of MMP-2 protein.Results After treatment 48 h,the cell proliferation rates of blank control group,experimental-L and-H groups were(38.07±2.53) %,(23.90±1.10) % and(12.00±2.65) %,the migration numbers was 683.33±110.15,322.00±72.19 and 132.33±18.23,the invasion number was715.33±109.70,300.00±60.67 and 135.00±30.64,the relative expressions of MMP-2 protein were 0.95±0.05,0.77±0.06 and0.27±0.06,respectively.And the differences were statistically significant between blank control group and experimental-L and-H groups(P <0.05 or P <0.01 or P <0.001).Conclusion Irigenin may inhibit the proliferation,migration and invasion of LN229 cells by inhibiting the expression of MMP-2.

关 键 词：野鸢尾黄素 人脑胶质瘤细胞LN229 细胞增殖 迁移 侵袭 作用机制 

分 类 号：R28[医药卫生—中药学]