LC-MS/MS法测定重症感染患者血浆中比阿培南浓度  

Determination of biapenem in human plasma of patients with severe infection by LC-MS/MS

作　　者：胡星星 程敏毓[1] 李力[1] HU Xing-xing;CHENG Min-yu;LI Li(Department of Pharmacy,Zhejiang Hospital,Hangzhou 310013,Zhejiang Province,China)

机构地区：[1]浙江医院药剂科,浙江杭州310013

出　　处：《中国临床药理学杂志》2021年第19期2684-2686,共3页The Chinese Journal of Clinical Pharmacology

基　　金：国家科技重大专项基金资助项目(2013ZX09303005);浙江省自然科学基金资助项目(LQ15H310003)。

摘　　要：目的建立一种测定人血浆中比阿培南浓度LC-MS/MS法,并应用于重症感染患者血浆中浓度测定。方法血浆样品处理用乙腈沉淀蛋白,色谱柱为Agilent Eclipse Plus C18(2.1 mm×100.0 mm,3.5μm),流动相为乙腈-含0.05%甲酸水,流速为0.30 mL·min^(-1),梯度洗脱。电喷雾离子源,正离子多反应监测,分析时间为3.5 min。考察该方法的专属性、标准曲线与定量下限、精密度与回收率、基质效应和稳定性。结果血浆中比阿培南在0.02~25.00μg·mL^(-1)内,线性关系良好(r=0.997 9),定量下限为0.02μg·mL^(-1);质控样品的相对误差(RE)在-9.35%~6.65%,日内、日间RSD均小于15%,提取回收率78.45%~86.18%,无明显基质效应,稳定性良好。结论该方法快速、灵敏、专属性强、重复性好,适用于人血浆中比阿培南的测定。Objective To establish a LC-MS/MS method for the determination of biapenem in plasma of patients with severe infection.Methods The plasma samples were precipitated by acetonitrile.The separation was performed on Agilent Eclipse Plus C18 column(2.1 mm×100.0 mm,3.5 μm) and eluted with acetonitrile-water(containing0.1% formic acid) using a gradient elution mode at a flow rate of 0.30 mL·min^(-1).Detection of the analyte was achieved using positive electrospray ionization with multiple reaction monitoring mode.The whole analytical time was 3.5 min.The specificity,standard curve,lower limit of quantitation,precision,recovery rate and stability as well as the matrix effect were investigated.Results The linear standard concentration range of biapenem was 0.02-25.00 μg·mL^(-1)(r=0.997 9),with the lower limit of quantitation of 0.02 μg·mL^(-1).Relative error was-9.35%-6.65%,Intra-day and inter-day relative standard deviations were both below 15%.The absolute recovery in plasma was78.45%-86.18%,no significant matrix effect and good stability was found.Conclusion The established method is rapid,sensitive,specific and reliable,suitable for the determination of biapenem in human plasma.

关 键 词：比阿培南 液质联用法 血药浓度 

分 类 号：R978.1[医药卫生—药品]