高效液相色谱法测定电解液中尿素的含量  

Determination of urea in electrolyte by HPLC

作　　者：刘洋[1] 王意[1] 黄艳梅 孟楠楠[2] 于一夫[2] LIU Yang;WANG Yi;HUANG Yanmei;MENG Nannan;YU Yifu(Analysis and Testing Center of Tianjin University,Tianjin 300072;Institute of Molecular Plus of Tianjin University,Tianjin 300072)

机构地区：[1]天津大学分析测试中心,天津300072 [2]天津大学分子+研究院,天津300072

出　　处：《分析试验室》2021年第10期1216-1218,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金面上项目(22071173)资助。

摘　　要：建立了高效液相色谱法测定电解液中微量尿素的方法,分别研究了测试过程中流动相配比、流速以及干扰物质(OH-,NO2-,HCOO-,CH3COO-,NH3,CH3OH,CH3CH2OH等)对尿素检测的影响。通过改变流动相不同配比、流速,进而调节尿素的出峰时间、面积,发现当流动相乙腈:水配比为40:60(V/V),流速为0.15 m L/min时,尿素出峰面积最大,且干扰物质与尿素的HPLC峰分离明显对尿素的检测无明显干扰。在最佳HPLC检测条件下,HPLC能够完成0~40 mg/L范围内的低浓度尿素含量检测。The content of urea in the electrolyte was determined by high performance liquid chromatography,and the effects of different component ratio of the mobile phase,flow rate,and interfering substances (OH-,NO2-,HCOO-,CH3COO-,NH3,CH3OH,CH3CH2OH,etc.) on the detection of urea were studied.The retention time and the peak area of urea could be changed by adjusting the composition and the flow rate of the mobile phase.The peak area of urea could reach the maximum value when the composition and the flow rate of mobile phase were 40:60 (CH3CN∶H2O,V/V) and 0.15 m L/min,respectively,and the HPLC peak of urea could be separated with interfering substances.Under the optimized HPLC conditions,detection of urea at a low concentration range from 0 to 40 mg/L could be achieved.

关 键 词：高效液相色谱法 电合成 尿素 

分 类 号：O657.7[理学—分析化学]