PCR荧光探针技术在宁波地区结核快速诊断中的应用价值分析  

The value of PCR fluorescence probe in the rapid diagnosis of tuberculosis in Ningbo region

作　　者：林律[1] 平国华[1] 车洋[1] 陈同[1] 杨天池[1] 周尧东 LIN Lv;PING Guo-hua;CHE Yang;CHEN Tong;YANG Tian-chi;ZHOU Yao-dong(Ningbo Municipal Center for Disease Control and Prevention,Ningbo,Zhejiang 315010,China)

机构地区：[1]宁波市疾病预防控制中心,浙江宁波315010 [2]中国科学院大学宁波华美医院(宁波市第二医院),浙江宁波315010

出　　处：《中国卫生检验杂志》2021年第19期2345-2347,共3页Chinese Journal of Health Laboratory Technology

基　　金：宁波市自然科学基金(20I8A610405.2019A610385)。

摘　　要：目的 探讨PCR荧光探针技术在我国东南沿海宁波地区的诊断价值。方法 选取2019年8月—2020年2月在中国科学院大学宁波华美医院肺科就诊的1 405例疑似肺结核患者为研究对象,对患者的痰标本先进行抗酸染色法检测后再分别进行BACTEC MGIT 960(MGIT 960)液体培养,并对培养阳性物进行结核分枝杆菌抗原检测,同时对痰液标本进行PCR荧光探针法检测。以抗酸染色法为基准,对PCR荧光探针技术和MGIT 960液体培养技术进行比较。结果 PCR荧光探针技术在宁波地区肺结核疑似患者中的阳性检出率(16. 2%)高于MGIT 960液体培养结合胶体金法(13. 1%)(P <0. 05),尤其在涂片阴性的疑似患者中,PCR荧光探针技术的阳性检出率(11. 6%)显著高于MGIT 960液体培养技术(7. 2%)(P <0. 01)。结论 PCR荧光探针技术对快速诊断结核病及提高宁波地区结核病发现率具有一定的积极意义。Objective To detect the value of PCR fluorescence probe assay in the rapid diagnosis of tuberculosis in Ningbo region.Methods A total of 1405 suspected tuberculosis patients admitted to the Department of Pulmonary Medicine,Ningbo Huamei Hospital,University of Chinese Academy of Sciences from August 2019 to February 2020 were selected as the research subjects.The sputum samples of the patients were tested by acid-fast staining and then BACTEC MGIT 960(MGIT 960)liquid culture.The positive culture samples were conducted for the test of Mycobacterium tuberculosis antigen,and the sputum samples were tested by PCR fluorescence probe.Based on acid fast staining method,PCR fluorescence probe technique was compared with MGIT 960 liquid culture technique.Results The positive detection rate of PCR fluorescence probe technology in suspected tuberculosis patients in Ningbo region(16.2%)was higher than that of MGIT 960 liquid culture combined with colloidal gold(13.1%)(P<0.05),especially in suspected patients with negative smear.The positive rate of PCR fluorescence probe(11.6%)was significantly higher than that of MGIT 960 liquid culture(7.2%)(P<0.01).Conclusion It may be a new way of using PCR fluorescence probe assay in the rapid diagnosis of tuberculosis in Ningbo to find more tuberculosis patients.

关 键 词：结核分枝杆菌 PCR 培养 诊断 

分 类 号：R446.5[医药卫生—诊断学]