基于叶绿体DNA条形码的竹子种属聚类分析与耐盐性鉴定  

Species clustering and salt tolerance identification of Bambusoideae by chloroplast DNA barcoding

作　　者：陈咸吉 聂现辉 艾文胜[3] 孟勇[3] 肖扬波 周楚人 彭逸斯 汪启明[1,2] 彭国平 CHEN Xianji;NIE Xianhui;AI Wensheng;MENG Yong;XIAO Yangbo;ZHOU Churen;PENG Yisi;WANG Qiming;PENG Guoping(College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha,Hunan 410128,China;Hunan Engineering Laboratory for Good Agricultural Practice and Comprehensive Utilization of Famous-Region Medicinal Plants,Changsha,Hunan 410128,China;Institute of Bamboo Research,Hunan Academy of Forestry,Changsha,Hunan 410011,China)

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]道地药用植物规范栽培与综合利用湖南省工程实验室,湖南长沙410128 [3]湖南省林业科学院竹类研究所,湖南长沙410011

出　　处：《湖南农业大学学报（自然科学版）》2021年第5期514-522,共9页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家重点研发计划项目(2018YFD0600102)。

摘　　要：利用3种叶绿体DNA条形码序列ndhF、psbA–trnH、rps16对竹亚科植物17个属的98个竹种进行DNA条形码聚类分析,并对已知的43个耐盐竹种进行聚类分析。结果显示:3种DNA条形码序列对竹子具有良好的通用性,平均通用性在96%以上;psbA–trnH未能对瓜多竹外的竹亚科植物种属进行聚类,能对部分耐盐竹种进行聚类;ndhF、rps16条形码序列能对部分种属进行聚类,对牡竹属、箣竹属、苦竹属中包含的耐盐竹种聚类效果较好。In this study,we used three chloroplast DNA barcoding,ndhF,psbA-trnH and rps16,for cluster analysis of 98 bamboo species belonging to 17 genera collected from Fujian Province plant garden and then performed the analysis on the 43 known salt tolerant bamboo species.The results showed that the used three DNA barcodes had good generality for bamboos with average generality of above 96%.The psbA-trnH could not cluster the species and genera of Bambusoideae other than Guadua,but could cluster some salt tolerant bamboo species.The ndhF and rps16 barcode sequences could cluster some species and genera,and the clustering effect of salt tolerant bamboos in Dendrocalamus,Bambusa and Pleioblastus was better.

关 键 词：竹亚科植物 DNA条形码 NJ系统发育树 耐盐性 

分 类 号：Q943.2[生物学—植物学] S795[农业科学—林木遗传育种]