丛枝菌根对干旱胁迫下油用牡丹幼苗光化学活性的影响  

Effect of arbuscular mycorrhiza on photochemical activity of oil tree peony seedlings under drought stress

作　　者：张文科 宋程威[1] 魏冬峰 石兆勇[1] 侯小改[1] ZHANG Wenke;SONG Chengwei;WEI Dongfeng;SHI Zhaoyong;HOU Xiaogai(College of Agriculture,Henan University of Science and Technology,Luoyang,Henan 471023,China;Luoyang Vocational College of Science and Technology,Luoyang,Henan 471822,China)

机构地区：[1]河南科技大学农学院,河南洛阳471023 [2]洛阳科技职业学院马克思主义学院,河南洛阳471822

出　　处：《湖南农业大学学报（自然科学版）》2021年第5期523-529,共7页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(U1804233);河南省教育厅项目(19A2180002)。

摘　　要：采用盆栽法,对重度干旱(土壤相对含水量为30%)和轻度干旱(土壤相对含水量为55%)胁迫3 d和6 d的接种丛枝菌根真菌(Gigaspora rosea)的油用牡丹‘凤丹’幼苗叶片叶绿素荧光参数进行测定。结果显示:与未接菌相比,干旱胁迫下接菌‘凤丹’的PSII潜在活性F_(v)/F_(o)和性能指数PI_(abs)均升高;重度干旱胁迫3、6 d,接菌‘凤丹’的M_(o)和V_(j)较未接菌处理分别显著降低14.5%、29.8%和12.9%和16.2%,φ_(Eo)和φ_(o)分别显著提高9.7%、52.2%和8%、23.6%;与未接菌相比,轻度干旱胁迫3、6 d,接菌‘凤丹’的单位面积吸收的光能ABS/CS_(o)、单位面积捕获的光能Tr_(o)/CS_(o)、单位面积电子传递的量子产额ETo/CSo、单位面积传递到PSⅠ末端的量子产额RE_(o)/CS_(o)均提高,但不显著;重度干旱胁迫3、6 d,接菌‘凤丹’的Tr_(o)/CS_(o)、ET_(o)/CS_(o)和单位面积反应中心数RC/CSm分别显著提高37.8%、41.1%、47.7%、73.8%、50.1%、141.8%;干旱胁迫下,接菌‘凤丹’的调节性能量耗散量子产量Y(NPQ)均显著高于未接菌处理,非调节性能量耗散量子产量Y(NO)显著低于未接菌处理。表明,丛枝菌根可以提高干旱胁迫下(尤其是重度干旱下)PSII反应中心活性,增加对光能的吸收和捕获,提高电子传递能力和光化学效率,同时将剩余的光能以热耗散的形式散发,从而避免光损伤,进而提高干旱胁迫下油用牡丹幼苗的光化学活性和抗旱性。The chlorophyll fluorescence parameters of potted oil peony‘Fengdan’seedlings inoculated with arbuscular mycorrhizal fungus Gigasporarosea under severe drought(soil relative water content is 30%)and mild drought(soil relative water content is 55%)stress for 3 and 6 days were compared and analyzed.The results showed that the potential PSⅡ activity F_(v)/F_(o) and performance index PI_(abs) of‘Fengdan’seedlings inoculated with arbuscular mycorrhiza were increased under different drought stress compared with the non-inoculated seedlings.Compared with the non-inoculated treatment,in inoculated‘Fengdan’with 3 and 6 days under severe drought stress,Mo significantly decreased by 14.5%and 29.8%respectively;Vj significantly decreased by 12.9% and 16.2%respectively;φ_(Eo) significantly increased by 9.7%and 52.2%respectively;and φ_(o) significantly increased by 8%and 23.6%,respectively.Compared with the non-inoculated treatment,in inoculated‘Fengdan’with mild drought stress for 3days and 6 days,absorption of energy per unit area(ABS/CS_(o)),capture of light energy per unit area(Tr_(o)/CS_(o)),quantum yield electron transfered per unit area(ET_(o)/CS_(o)),quantum yield transfered at the end of PSⅠper unit area(RE_(o)/CS_(o))were improved,but with no significant difference.Compared with the non-inoculated treatment,in inoculated‘Fengdan’with under severe drought stress for 3 and 6 days,Tr_(o)/CS_(o) were significantly increased by 37.8%and 41.1%respectively,ET_(o)/CS_(o) significantly increased by 47.7%and 73.8%respectively,and the number of response centers per unit area(RC/CS_(m))significantly increased by 50.1%and 141.8%,respectively.Under drought stress,regulatory energy dissipation quantum yield Y(NPQ)of inoculated‘Fengdan’was significantly higher than that of non-inoculated‘Fengdan’,and non-regulatory energy dissipative quantum yield Y(NO)was significantly lower than that of non-inoculated.The research showed that arbuscular mycorrhizal can improve PSⅡ reaction center activity under t

关 键 词：油用牡丹 叶绿素荧光 PSⅡ反应中心活性 光化学效率 丛枝菌根 干旱胁迫 

分 类 号：S682.190.1[农业科学—观赏园艺]