大雅柑枯斑病斑的病原鉴定及防治药剂的筛选  

Identification of Stagonosporopsis cucurbitacearum as the pathogen of citrus and screening of its fungicides

作　　者：占爽 杨静 胡军华[1,3] 吴玉珠 吴望 张嘉 陈娜 周彦 ZHAN Shuang;YANG Jing;HU Junhua;WU Yuzhu;WU Wang;ZHANG Jia;CHEN Na;ZHOU Yan(Citrus Research Institute,Southwest University,Chongqing 400712,China;College of Plant Protection,Southwest University,Chongqing 400712,China;Fruit Tree Science Observation and Experimental Station in Southwest China,Ministry of Agriculture,Chongqing 400712,China)

机构地区：[1]西南大学柑桔研究所,重庆400712 [2]西南大学植物保护学院,重庆400712 [3]农业部西南地区果树科学观测实验站,重庆400712

出　　处：《湖南农业大学学报（自然科学版）》2021年第5期535-539,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：科技部国家重点研发计划项目(2017YF D0202006–04);农业部国家现代农业柑橘产业技术体系(CARS–26–05B)。

摘　　要：2020年春季,于重庆市长寿区柑橘园内采集大雅柑典型枯斑病果实,采用组织块分离法分离得到菌株CS11,对其进行形态学和分子生物学鉴定,确定CS11为Stagonosporopsis cucurbitacearum。向健康的柑橘盆钵苗喷洒CS11孢子悬液可致植株患病,从患病样品中分离得到菌株CS11,确认CS11是致病病原菌;CS11对大雅柑(Citrus reticulata.cv.dayagan)、有核沃柑(Citrus rtemple×Dancy)、塔罗科血橙(Tarocco blood orange(Citrus sinensis(L.)Osbeck))和尤力克柠檬(Citrus limon)的叶、枝、果实均具有致病性。室内药剂筛选结果显示,75%肟菌·戊唑醇水分散粒剂、60%唑醚·代森联可湿性粉剂、450 g/L咪鲜胺乳油、37%苯醚甲环唑可湿性粉剂、11.5%吡唑嘧菌酯乳油5种杀菌剂对CS11有较好的抑制作用,其中75%肟菌·戊唑醇水分散粒剂、60%唑醚·代森联可湿性粉剂、37%苯醚甲环唑可湿性粉剂、11.5%吡唑嘧菌酯乳油等4种药剂的EC50值均小于10 mg/kg。In the spring of 2020,the typical diseased citrus fruits were collected in Changshou,Chongqing,and the blight fruits of dayagan(Citrus reticulate cv.dayagan)were obtained.The strain CS11 was obtained from the diseased samples by tissue separation.Through morphology and molecular biology characterization,strain CS11 was determined as Stagonosporopsis cucurbitacearum(S.ucurbitacearum).Pathogenicity study by spraying the spores on health citrus plants showed that strain CS11 caused blight on citrus,which confirmed the pathogenicity of strain CS11.S.ucurbitacearum strain CS11 has pathogenicity on Citrus reticulate cv.Dayagan,C.rtemple×Dancy,C.sinensis(L.)Osbeck(Tarocoo blood orange)and C.limon.And the strain CS11 is pathogenic to the leaves,branches and fruits of the 4 kinds of citrus.The growth rate method was used to determine the virulence of different agents to the strain,the results showed five fungicides including 75%trifloxystrobin tebuconazole,60%pyraclostrobin metiram,450 g/L prochloraz,37%difenoconazole and 11.5%pyraclostrobin had good inhibitory effect on strain CS11.The EC50 values of 75%trifloxystrobin tebuconazole,60% pyraclostrobin metiram, 37% difenoconazole and 11.5% pyraclostrobin were all less than 10 mg/kg.

关 键 词：大雅柑 枯斑病 病原鉴定 致病性 药剂筛选 

分 类 号：S436.661.19[农业科学—农业昆虫与害虫防治]