高效液相色谱法测定有机-无机复合肥料中4种抗生素的含量  

Determination of the Content of Four Antibiotics in Organic-Inorganic Compound Fertilizer by HPLC

作　　者：邓帆 龚宁 DENG Fan;GONG Ning(Guizhou Institute of Products Quality Inspection and Testing,Guiyang,Guizhou 550014,China)

机构地区：[1]贵州省产品质量检验检测院,贵州贵阳550014

出　　处：《肥料与健康》2021年第5期74-76,共3页Fertilizer & Health

摘　　要：建立了高效液相色谱法测定有机-无机复合肥料中土霉素、四环素、金霉素、强力霉素等4种抗生素残留的方法。有机-无机复合肥料样品经乙二胺四乙酸二钠-Mcllvaine-甲醇提取后,以EC-C 18色谱柱为固定相,以不同体积比的甲醇-乙腈(1+1)和0.01 mol/L草酸混合溶液为流动相进行梯度洗脱,外标法定量。结果表明:4种抗生素的质量浓度在0.1~20.0μg/mL内与其峰面积呈线性关系,检出限为0.75~1.0 mg/kg,加标回收率为96.7%~104%,测定值的相对标准偏差为1.6%~2.7%。A method for determination of four antibiotic residues in organic-inorganic compound fertilizer,including oxytetracycline,tetracycline,chlortetracycline,and doxycycline,by high performance liquid chromatography(HPLC)is established.The organic-inorganic compound fertilizer sample is extracted by EDTA disodium-Mcllvaine-methanol,gradient eluted with EC-C 18 column as stationary phase and mixed solution of methanol-acetonitrile(1+1)and 0.01 mol/L oxalic acid in different volume ratios as mobile phase and quantified by external standard method.The results show that the mass concentrations of the 4 antibiotics have linear relationship with their peak areas within 0.1-20.0μg/mL,the detection limit is 0.75-1.0 mg/kg,the recovery rate is 96.7%-104%,and the relative standard deviation of measured values is 1.6%-2.7%.

关 键 词：高效液相色谱法 抗生素 有机-无机复合肥料 测定 

分 类 号：O657.72[理学—分析化学]