基于非靶向代谢组学的茄梨和红茄梨成熟期果皮代谢产物的差异分析  

Analysis of metabolite differences in skin between Clapp’s Favorite and its mutant Red Clapp’s Favorite through non-targeted metabolomics

作　　者：牟红梅[1] 慈志娟[1] 艾沙江·买买提[2] 梁艳萍 刘笑宏 杜晓云 于强[1] 李庆余[1] 李元军[1] MU Hongmei;CI Zhijuan;Aisajan MAMAT;LIANG Yanping;LIU Xiaohong;DU Xiaoyun;YU Qiang;LI Qingyu;LI Yuanjun(Yantai Academy of Agricultural Sciences Shandong Province, Yantai 26550, China;Xinjiang Academy of Agricultural Sciences, Urumchi 830091, China;Yunnan Academy of Agricultural Sciences, Yunnan 650205, China)

机构地区：[1]山东省烟台市农业科学研究院,山东烟台265500 [2]新疆农业科学院,新疆乌鲁木齐830091 [3]云南省农业科学研究院,云南昆明650205

出　　处：《色谱》2021年第11期1203-1212,共10页Chinese Journal of Chromatography

基　　金：财政部和农业农村部国家现代农业产业技术体系;中央财政林业科技推广示范补贴项目.

摘　　要：为了探究西洋梨品种茄梨及其红色芽变红茄梨成熟期果皮代谢产物差异,采用超高效液相色谱-质谱联用技术,对茄梨和红茄梨成熟期果皮进行非靶向代谢组学研究。通过主成分分析和正交偏最小二乘判别分析,构建了多变量统计分析模型,结合模型和变量重要性投影与最大差异倍数值,基于精确质量数、二级碎片以及同位素分布,使用PMDB(Plant Metabolome Database)数据库进行定性,筛选并鉴定出茄梨和红茄梨果皮中显著性变化(P<0.05,VIP(variable importance in project)≥1)的差异代谢物有83种,主要包括酚酸类、黄酮类和氨基酸类物质,涉及类黄酮代谢、氨基酸代谢、苯丙烷类代谢等代谢途径,其中53种物质含量上调,30种物质表达下调。通过KEGG(Kyoto Encyclopedia of Genes and Genomes)数据库进一步对差异代谢物质进行通路富集分析,差异代谢物主要分布在20条代谢途径中,P<0.05的代谢途径有6条,分别是类黄酮生物合成、黄酮和黄酮醇生物合成、苯丙烷生物合成、丁酸酯代谢、苯丙氨酸代谢、酪氨酸代谢。这些差异代谢物的变化可能是导致茄梨和红茄梨果皮色泽不同的原因。该研究从植物代谢组学角度初步揭示了茄梨和红茄梨成熟期果皮的代谢产物差异性。Red Clapp’s Favorite is the red mutation cultivar of the pear cultivar Clapp’s Favorite.Fruit color is an important feature of pear fruits,with red skin generally attracting consumers.Anthocyanin,chlorophyll,and carotenoids are the most important pigments in the color formation of fruits.The red color of pear skin is mainly due to the concentration and composition of anthocyanin.Metabolomics is an emerging discipline that focuses on the qualitative and quantitative analysis of small metabolites with low molecular weight in biological cells and tissues.As an important part of systems biology,it is an effective means to solve many complex biological problems.Studies have analyzed pigment content,composition,and differentially expressed genes in the skin of green and red pears from various aspects.Anthocyanins are responsible for physiological activity on regulating pathways.The aim of this study was to discover differential metabolites in the skin of Clapp’s Favorite and its red mutation cultivar Red Clapp’s Favorite.The metabolic components were detected using high-performance liquid chromatography coupled with tandem mass spectrometry(UPLC-MS/MS).Chromatographic experiments were performed on an HSS T3 column(100 mm×2.1 mm,1.8μm)by using a mobile phase consisting of 0.1%(v/v)formic acid in acetonitrile and water,and mass spectrometry was conducted in the positive and negative modes by electrospray ionization(ESI).Red Clapp’s Favorite and Clapp’s Favorite were collected from the pear germplasm resource nursery of Yantai Institute of Agricultural Sciences in Shandong.The data were analyzed by principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)as well as cluster analysis and heat map.The first two principal components exhibited 62.3%and 8%of the total variance in the positive and negative ion modes,respectively.PCA can generally reflect metabolite differences between the two groups of samples,and there are significant differences in metabolites between th

关 键 词：超高效液相色谱-质谱 植物代谢组学 差异代谢物 梨 果皮 

分 类 号：O658[理学—分析化学]