变异型PEDV北京分离株ELISA检测法的建立  

Establishment of ELISA detection method for Beijing strains of variant PEDV

作　　者：夏婷 郭娇娇 廖晨星 阮文科[1] XIA Ting;GUO Jiaojiao;LIAO Chenxing;RUAN Wenke(College of Animal Science and Technology,Beijing University of Agriculture,Beijing 102206,China)

机构地区：[1]北京农学院动物科学技术学院,北京102206

出　　处：《北京农学院学报》2021年第4期81-86,共6页Journal of Beijing University of Agriculture

摘　　要：【目的】为基层养殖场的批量样本检测以及猪流行性腹泻病(PED)的快速诊断提供更为有效的方法。【方法】利用原核表达系统表达了猪流行性腹泻病毒(PEDV)S1部分基因序列,建立用于检测变异型PEDV北京分离株的间接酶联免疫吸附测定方法。【结果】经试验检测表明,该ELISA检测方法敏感性较高、重复性良好、特异性显著、有良好的稳定性和较长的保存期,且临床样本检测与市场上试剂盒比较符合率较高。【结论】此方法可以作为临床免疫检测变异株PEDV的方法,为PEDV抗体检测试剂盒和基因工程疫苗的开发提供了依据。【Objective】It provides a more effective method for batch sample detection and rapid diagnosis of porcine epidemic diarrhea disease(PED)in grass-roots farms.【Methods】Part of gene sequences of porcine epidemic diarrhea virus(PEDV)S1 were expressed by prokaryotic expression system,and an indirect enzyme linked immunosorbent assay was established for the detection of Beijing strain of PEDV.【Results】The test results showed that the ELISA method had high sensitivity,good reproducibility,significant specificity,good stability and long shelf life,and the coincidence rate of clinical samples was higher than that of kits on the market.【Conclusion】This method can be used for clinical immunodetection of PEDV variant strains,and provides a basis for the development of PEDV antibody detection kit and genetic engineering vaccine.

关 键 词：猪 流行性腹泻 基因 原核表达 蛋白 间接酶联免疫吸附 

分 类 号：S852.65[农业科学—基础兽医学]