Intestinal lysozyme releases Nod2 ligand(s) to promote the intestinal mucosal adjuvant activity of cholera toxin  

作　　者：Haifang Wang Xueying Shen Xiaojiao Zheng Ying Pan Qin Zhang Zhihua Liu 

机构地区：[1]Institute for Immunology and School of Medicine,Tsinghua University,Beijing 100084,China [2]Key Laboratory of Infection and Immunity,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China [3]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Science China(Life Sciences)》2021年第10期1720-1731,共12页中国科学（生命科学英文版）

基　　金：supported by the National Key Research and Development Program of China(2017YFA0503403);the National Natural Science Foundation of China(31730028 and 81670482);the Key Program for Frontier Science of the Chinese Academy of Sciences(QYZDBSSW-SMC025).

摘　　要：Commensal bacteria boost serum IgG production in response to oral immunization with antigen and cholera toxin(CT)in a manner that depends on Nod2(nucleotide-binding oligomerization domain-containing protein 2).In this study,we examined the role of intestinal lysozyme(Lyz1)in adjuvant activity of CT.We found that Lyz1 released Nod2 ligand(s)from bacteria.Lyz1 deficiency reduced the level of circulating Nod2 ligand in mice.Lyz1 deficiency also reduced the production of IgG and T-cellspecific cytokines after oral immunization in mice.Supplementing Lyz1-deficient mice with MDP restored IgG production.Furthermore,overexpression of Lyz1 in intestinal epithelium boosted the antigen-specific IgG response induced by CT.Collectively,our results indicate that Lyz1 plays an important role in mediating the immune regulatory effect of commensal bacteria through the release of Nod2 ligand(s).

关 键 词：Paneth cell lysozyme 1 muramyl dipeptide cholera toxin NOD2 

分 类 号：R392[医药卫生—免疫学]