汉赛巴尔通体效应蛋白BepE与血管内皮细胞迁移的相关性研究  

The Effect on HUVEC Migration Induced by T4SS Effector BepE of Bartonella henselae

作　　者：蔡雨豪 王蒙 王春艳[1] 丁田耘 张浩然[1] 袁聪俐[1] CAI Yuhao;WANG Meng;WANG Chunyan;DING Tianyun;ZHANG Haoran;YUAN Congli(School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海交通大学农业与生物学院,上海200240

出　　处：《中国动物传染病学报》2021年第5期1-6,共6页Chinese Journal of Animal Infectious Diseases

基　　金：国家自然科学基金(31972653)。

摘　　要：本研究利用SacB介导的同源重组技术构建汉塞巴尔通体效应蛋白BepE的缺失突变菌株Bhe-ΔBepE以及效应蛋白BepE的回补表达菌株Bhe-pBepE。将Bhe、Bhe-ΔBepE与Bhe-pBepE分别感染人血管内皮细胞(HUVEC),24 h后进行细胞划痕实验,在0 h、6 h、12 h与18 h对划痕区域进行定点拍摄,通过计算细胞迁移率比较三种菌感染后的HUVEC迁移能力。结果显示,3种菌感染后的HUVEC迁移能力并无显著区别,即Bhe效应蛋白BepE并没有促进血管内皮细胞迁移的作用。SacB-mediated in-frame deletion strategy was used here to construct the knock out strain Bartonella henselae(Bhe-ΔBepE),a type 4 secretion system effector.Bhe-BepE complemented in trans in Bhe-ΔBepE strain was also constructed for comparison.Subsequently,human vascular endothelial cells(HUVECs)were infected by wild type Bhe,Bhe-ΔBepE and Bhe-pBepE for 24 hours and then wounds were produced by needle scratch.Wound healing was determined at the time of 0 h,6 h,12 h and 18 h.The results showed that there was no signifi cant difference of the migration ability of HUVECs after the infection of wild type Bhe,Bhe-ΔBepE and Bhe-pBepE,indicating BepE of Bhe failed to promote the migration of HUVECs.

关 键 词：汉赛巴尔通体 效应蛋白BepE 基因敲除 细胞划痕 

分 类 号：S852.64[农业科学—基础兽医学]