Flexible dopamine-sensing fiber based on potentiometric method for long-term detection in vivo  

作　　者：Liyuan Wang Jiawei Chen Jiajia Wang Hongjian Li Chuanrui Chen Jianyou Feng Yue Guo Hongbo Yu Xuemei Sun Huisheng Peng 

机构地区：[1]State Key Laboratory of Molecular Engineering of Polymers,Department of Macromolecular Science,and Laboratory of Advanced Materials,Fudan University,Shanghai 200438,China [2]Vision Research Laboratory,School of Life Sciences,State Key Laboratory of Medical Neurobiology,Collaborative Innovation Centre for Brain Science,Fudan University,Shanghai 200438,China

出　　处：《Science China Chemistry》2021年第10期1763-1769,共7页中国科学（化学英文版）

基　　金：the National Natural Science Foundation of China(22075050,21634003);the Ministry of Science and Technology of China(2016YFA0203302);the Science and Technology Commission of Shanghai Municipality(20JC1414902);Shanghai Municipal Education Commission(2017-01-07-00-07-E00062)。

摘　　要：Achieving real-time,continuous and long-term monitoring of dopamine(DA)in vivo is essential for revealing brain functions and preventing and treating neurogenic diseases.However,it remains challenging to achieve a low limit of detection(LOD)and high neuron-compatibility at the same time for the current microsensors,resulting in the failure of long-term and accurate detection of DA in vivo.A DA-sensing fiber was achieved by the potentiometric method to possess a low LOD of 5 nM,1-3 orders of magnitude lower than amperometry and differential-pulse voltammetry.The sensing fiber showed a wide linear range from 5 to 185 nM that well matched the DA concentration(26-40 nM)in vivo.After implantation,the sensing fiber showed no influence on the firing rates of neurons with the potentiometric test,indicating high neuron-compatibility.It was then integrated with electrophysiology to simultaneously monitor DA variation and electrical signal in the brain,with stable monitoring of DA change in vivo for 8 weeks.The sensing fiber was flexible and stably worked after hundreds of bending,and it showed high sensitivity even after protein adsorption,thus offering a reliable tool for neuroscience.

关 键 词：carbon nanotube fiber POTENTIOMETRIC IMPLANTABLE LONG-TERM dopamine detection 

分 类 号：R318[医药卫生—生物医学工程] TQ342.742[医药卫生—基础医学]