大鼠腹内壁中线间质通道微观结构的活体激光共聚焦成像观察  

Observation of Microstructure of Midline Interstitial Channels of the Inner Abdominal Wall in Rat for in vivo Confocal Laser Imaging

作　　者：宋晓晶[1] 熊枫 贾术永[1] 王广军[1] 王淑友[1] 王瑾玉 张维波[1] SONG Xiaojing;XIONG Feng;JIA Shuyong;WANG Guangjun;WANG Shuyou;WANG Jinyu;ZHANG Weibo(Department of Biomedical Engineering,Institute of Acupuncture&Moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院针灸研究所,北京100700

出　　处：《激光生物学报》2021年第5期435-440,共6页Acta Laser Biology Sinica

基　　金：国家自然科学基金原创探索计划项目(82050006)。

摘　　要：为探究循经间质通道的微观结构,应用活体激光共聚焦成像系统对大鼠腹内壁腹中线,即循任脉间质通道荧光素钠聚积部位进行观察。通过使用荧光照相法对尾静脉注射荧光素钠的大鼠腹内壁表面的荧光素钠聚集轨迹定位,应用活体激光共聚焦成像系统观察腹内壁表面中线荧光素钠聚集轨迹上不同位点与旁开点组织的结构差异,并同离体组织的Masson染色结果比较。结果发现:腹内壁中线分布有大量与中线走行平行、有序排列的纤维组织,其中富含大量组织液;旁开为致密的肌纤维,表面的纤维排列不与中线平行。循任脉间质通道具有大量平行纤维结构分布的特征,为组织液向腹中线汇聚,并在循任脉间质通道中流动提供了组织形态学基础,为经络循行和结构的可视化提供了新的方法和证据,对于经络生物学内涵研究具有重要意义。In order to explore the microstructure of interstitial channels along meridians in vivo confocal laser imaging system is used to observe the midline of the inner abdominal wall which is the accumulation site of sodium fluorescein in interstitial channels along conception vessel in rats.The tracks of sodium fluorescein aggregation on the surface of inner abdominal wall of rats after sodium fluorescein injection via caudal vein is determined by fluorescence photography.In vivo confocal laser imaging system is used to observe the structural differences between different sites on the tracks of sodium fluorescein along the midline of inner abdominal wall and its adjacent open sites.The results are compared with those of the Masson staining in vitro.It is found that a lot of fibrous tissue parallels to the midline in the interstitial channel along the inner abdominal wall and arranges in order in rat,which is rich in tissue fluid.The fibers on the surface of side muscle tissues are not parallel to the midline.A large number of parallel fiber structures in the interstitial channels along the conception vessel provides a histomorphological basis for the convergence of interstitial fluid to the midline of abdominal wall and the interstitial flow along it.It provides a new method and evidence for the visualization of meridian running and structure.It is of great significance for the exploration of the biologi-cal connotation of meridians.

关 键 词：活体激光共聚焦成像 间质通道 腹内壁中线 纤维结缔组织 大鼠 

分 类 号：Q631[生物学—生物物理学]