拟康宁木霉对甲基橙废水的酶促降解研究  

A Study on Enzymatic Degradation of Methyl Orange Wastewater by Trichoderma koningii

作　　者：王海琪[1] 林斌 潘硕滨 林锦美[2] WANG Haiqi;LIN Bin;PAN Shuobin;LIN Jinmei(Jimei University College of Marine Food and Bioengineering,Xiamen 361021,China;Jimei University College of Port and Environmental Engineering,Xiamen 361021,China)

机构地区：[1]集美大学海洋食品与生物工程学院,厦门361021 [2]集美大学港口与环境工程学院,厦门361021

出　　处：《激光生物学报》2021年第5期468-474,共7页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(51004053);福建省教育厅资助项目(JT180289)。

摘　　要：利用驯化获得的拟康宁木霉菌(Trichoderma koningii)处理甲基橙废水,以脱色率为指标探讨了反应时间、初始甲基橙浓度、pH、葡萄糖用量、不同培养时间等培养因素对拟康宁木霉生长前期降解甲基橙废水效果的影响;在较优条件下以脱色率为指标,探讨了酶在pH、温度、酶液投加量、初始甲基橙浓度、反应时间等条件下的酶促降解反应;并通过正交试验验证其脱色降解甲基橙染料的最佳反应条件,为染料废水的处理提供试验基础。试验表明,培养过程中拟康宁木霉菌降解甲基橙废水的最适条件为初始甲基橙浓度30 mg/L,培养时间72 h,葡萄糖浓度25 g/L,pH 6.5。在较优培养条件下提取的酶,在酶液投加量为0.6 mL,温度为25℃,pH为7.0,处理时间为6 h的处理条件下,其降解率可达85.48%,脱色效果较明显。本研究为拟康宁木霉对甲基橙染料废水的酶促降解条件提供了参考。Methyl orange wastewater was treated by domesticated Trichoderma koningii.Taking the decolorization rate as the index,the effects of culture factors such as reaction time,initial methyl orange concentration,pH,glucose dosage and different culture time on the degradation of methyl orange wastewater in the early growth stage of Trichoderma koningii were discussed.Taking the decolorization rate as the index,the enzymatic degradation reaction under the condition of certain pH,temperature,enzyme solution dosage,initial methyl orange concentration and reaction time was discussed;the optimum conditions for decolorization and degradation of methyl orange dye were verified by orthogonal test,which provided an experimental basis for the biological treatment of dye wastewater.The results showed that the optimum condition for the degradation of methyl orange wastewater by Trichoderma koningii was initial methyl orange concentration of 30 mg/L,culture time of 72 h,glucose concentration of 25 g/L and pH of 6.5.The degradation rate of the enzyme extracted under the optimal culture condition can reach 85.48%under the treatment condition of enzyme solution dosage of 0.6 mL,temperature of 25℃,pH of 7.0 and treatment time of 6 h,the decolorization effect is obvious,which provides a reference for the enzymatic degradation condition of methyl orange dye wastewater by Trichoderma koningii.

关 键 词：拟康宁木霉 甲基橙废水 培养条件 酶促降解 脱色率 

分 类 号：X703[环境科学与工程—环境工程]