印度獐牙菜中[口山]酮类成分的UPLC-ESI-Q-TOF-MS分析  

UPLC-ESI-Q-TOF-MS Analysis of Xanthones in Swertia Chirayita

作　　者：董惠敏 许如玲 陈晓青 杨福燕 范君婷[3] 鞠建明[1,2] DONG Hui-min;XU Ru-ling;CHEN Xiao-qing;YANG Fu-yan;FAN Jun-ting;JU Jian-ming(The Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210028, China;Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, China;School of Pharmacy, Nanjing Medical University, Nanjing, 211166, China)

机构地区：[1]南京中医药大学附属中西医结合医院,江苏南京210028 [2]江苏省中医药研究院,江苏南京210028 [3]南京医科大学药学院,江苏南京211166

出　　处：《南京中医药大学学报》2021年第5期744-749,共6页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省“六大人才高峰”高层次人才选拔培养项目(2015-YY-011);江苏省“333高层次人才培养工程”项目(BRA2015494);江苏省卫生计生委医学科研课题(Z2018035);南京中医药大学自然科学基金(XZR2020021)。

摘　　要：目的建立印度獐牙菜中[口山]酮类成分的超高效液相色谱-电喷雾四极杆飞行时间质谱(UPLC-ESI-Q-TOF-MS)定性分析方法。方法选用Acquity HSS T3色谱柱(100 mm×2.1 mm,1.8μm),0.1%甲酸水-0.1%甲酸乙腈溶液作为流动相进行梯度洗脱,流速0.4 mL/min,柱温40℃。在正/负离子模式下采集印度獐牙菜醇提物的质谱数据,采用Masslynx4.1软件并结合SciFinder数据库、质谱数据、对照品信息和獐牙菜属植物相关文献进行数据分析。结果在印度獐牙菜中共鉴定出[口山]酮类化合物25个,其中3个成分为首次从印度獐牙菜中发现,分别是7-O-β-D-吡喃木糖-1,8-二羟基-3-甲氧基[口山]酮、Swerpunilactone B和8-O-[β-D-吡喃木糖-(1→6)-β-D-吡喃葡萄糖]-1,7-二羟基-3-甲氧基[口山]酮。结论研究为印度獐牙菜中[口山]酮类化学成分的定性分析及药材质量的控制提供实验依据。OBJECTIVE To establish a qualitative analysis method of xanthones in Swertia chirayita by ultra-high performance liquid chromatography-time-of-flight mass spectrometry(UPLC-ESI-Q-TOF-MS).METHODS Acquity HSS T3 column(100 mm×2.1 mm,1.8μm)was used as the chromatographic column,with 0.1%formic acid in water-0.1%formic acid in acetonitrile as the mobile phase for gradient elution.The volume flow rate was 0.4 mL·min-1 and the column temperature was 40℃.In the positive and negative ion modes,the mass spectrum data of Swertia chirayita was collected and identified by Masslynx4.1 software combined with SciFinder database,comparison with mass spectrometry data analysis,reference materials and reference to relevant literatures.RESULTS A total of 25 xanthone compounds were identified,3 of which were first discovered including 7-O-β-D-xylopyranosyl-1,8-dihydroxy-3-methoxyxanthone,swerpunilactone B and 8-O-[β-D-xylopyranose(1→6)-β-D-glucopyranose]-1,7-dihydroxy-3-methoxyxanthone.CONCLUSION The method can provide an experimental basis for the qualitative analysis of xanthones and the quality control of medicinal materials in Swertia chirayita.

关 键 词：印度獐牙菜 [口山]酮 UPLC-ESI-Q-TOF-MS 

分 类 号：R284[医药卫生—中药学]