基于雾化全氟化碳纳米探针的小鼠多核^(1)H/^(19)F-MR肺通气成像研究  

The study of multi-nuclide^(1)H/^(19)F-MR lung ventilation imaging based on atomized perfluorocarbon nanoprobe

作　　者：姜颖 陈静[1,2] 董婧 吴丽娜[1,2] JIANG Ying;CHEN Jing;DONG Jing;WU Lina(Department of Nuclear Medicine,the Fourth Hospital of Harbin Medical University,Harbin 150028,China;NHC and CAMS Key Laboratory of Molecular Probe and Targeted Theranostics,Molecular Imaging Research Center(MIRC),Harbin Medical University,Harbin 150028,China)

机构地区：[1]哈尔滨医科大学附属第四医院核医学科,哈尔滨150028 [2]哈尔滨医科大学分子影像研究中心,哈尔滨150028

出　　处：《磁共振成像》2021年第10期26-31,共6页Chinese Journal of Magnetic Resonance Imaging

基　　金：国家自然科学基金项目(编号:81771903);中央支持地方高校改革发展基金优秀青年人才项目(编号2020YQ04);哈尔滨医科大学第四临床医学院院内基金(编号:HYDSYJQ201601)。

摘　　要：目的探讨应用雾化全氟化碳纳米探针(perflurocarbon nanoprobe,PFC NP)进行多核^(1)H/^(19)F磁共振(^(1)H/^(19)F magnetic resonance,^(1)H/^(19)F-MR)肺通气成像的可行性。材料与方法将BALB/c鼠分为空气组和雾化PFC NP组,并进行多核^(1)H/^(19)F-MR扫描,研究经呼吸道递送雾化PFC NP后肺内^(19)F磁共振(^(19)F magnetic resonance,^(19)F-MR)信号的分布;在0 h、1 h、4 h、8 h、12 h、24 h、48 h分别进行^(1)H/^(19)F-MR扫描,研究雾化PFC NP在肺内的代谢规律;通过组织切片验证雾化PFC NP在肺组织中的沉积和对主要脏器的影响。结果雾化PFC NP能够使双肺^(19)F-MR信号明显增强,且肺各区域的^(19)F信号强化程度不同,其中上肺野和下肺野之间、中肺野和下肺野之间的^(19)F信号强度差异有统计学意义(P<0.01);各时间点^(1)H/^(19)F-MR成像发现,^(19)F-MR信号在递送雾化PFC NP后8 h开始减弱,24 h显著减弱,48 h减至背景信号水平;组织切片证实经呼吸道递送后PFC NP在肺组织沉积,H&E染色显示经呼吸道递送雾化PFC NP后各组织未见病理损伤。结论基于雾化PFC NP的多核^(1)H/^(19)F-MR肺通气成像能够反映肺解剖结构及其生理状态下的肺通气信息,可以通过多核磁共振实现肺通气成像,在呼吸系统磁共振成像领域具有重要应用前景。Objective:To explore the feasibility of applying atomized perfluorocarbon nanoprobe(PFC NP)for multi-core^(1)H/^(19)F magnetic resonance(^(1)H/^(19)F-MR)lung ventilation imaging.Materials and Methods:The BALB/c mice were divided into air group and atomized PFC NP group to perform multi-core^(1)H/^(19)F-MR scanning to study the distribution of PFC NPs in the lungs.^(1)H/^(19)F-MR scans were performed at 0 h,1 h,4 h,8 h,12 h,24 h,and 48 h to study the metabolic time of the aerosolized PFC NPs in the lungs.To verify the deposition of aerosolized PFC NPs in lung tissues and their effects on major tissues and organs through pathological examinations.Results:The atomized PFC NPs can significantly enhance the^(19)F magnetic resonance(^(19)F-MR)signal of bilateral lungs.And it was observed that the^(19)F signal enhancement in different lung fields is different.Among them,the^(19)F signal intensity difference between the upper and the lower lung fields,and between the middle and the lower lung fields is statistically significant(P<0.01).By performing^(1)H/^(19)F-MR imaging at each time point,it was found that the^(19)F signal began to attenuate at 8 h after the PFC NPs was delivered,significantly decreased at 24 h,and declined to the signal intensity of background at 48 h.Tissue sections confirmed the deposition of PFC NPs in lung tissue after delivery through the respiratory tract.H&E staining showed that no pathological damage was observed indifferent organs after the atomized PFC NP was delivered through the respiratory tract.Conclusions:Multi-core^(1)H/^(19)F-MR lung ventilation imaging based on atomized PFC NP can present the pulmonary anatomy and its ventilation information under physiological conditions.It is promising in the field of respiratory system magnetic resonance imaging.

关 键 词：肺通气 肺成像 全氟化碳 多核磁共振 19F磁共振 

分 类 号：R445.2[医药卫生—影像医学与核医学] R563[医药卫生—诊断学]