检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:宋科昕 张婕 王恺悦 邢佳 符园园 邓思琪 邹宇 顾玲[1] 翟效月[1] SONG Ke-xin;ZHANG Jie;WANG Kai-yue;XING Jia;FU Yuan-yuan;DENG Si-qi;ZOU Yu;GU Ling;ZHAI Xiao-yue(Department of Histology and Embryology,College of Basic Medical Science,China Medical University,Shenyang 110122,China)
机构地区:[1]中国医科大学基础医学院组织学胚胎学教研室,沈阳110122
出 处:《解剖学报》2021年第5期784-788,共5页Acta Anatomica Sinica
基 金:国家自然科学基金(31971115)。
摘 要:目的根据肾脏发生发育规律,探讨毛细血管袢期肾小球(cap G)中毛细血管的体积密度变化规律。方法取发生发育多时间点的胚胎及生后早期小鼠肾脏,制备石蜡切片,采用内皮细胞标记物CD34免疫组织化学染色,结合体视学网格系统,测量CD34阳性表达的内皮细胞及其围成的毛细血管占肾小球的体积密度。结果根据肾小球的形态学及足细胞排列形式,将cap G分为早、中、晚3期,cap G中期又分为中早期和中晚期。除cap G早期由于肾小球形状复杂无法测量外,中早期内皮细胞阳性的毛细血管袢的体积密度为(35.95±6.45)%,中晚期为(58.36±6.30)%,cap G晚期体积密度为(79.89±5.21)%,作为对照的成熟期为(93.61±1.96)%。此外,不同发育时间点及相同发育期的肾小球毛细血管袢的体积密度保持恒定。结论肾小球发育过程中反映其有效滤过面积的毛细血管袢占肾小球的比例呈显著递增趋势,该结果为肾小球发育的评估提供了参数依据。Objective To describe quantitatively the development of the capillary loop stage glomerulus(capG) with respect to the volume density of capillaries in the glomerulus based on the morphogenesis of the kidney.Methods The kidneys were obtained from mice at various developing time points and prepared for paraffin sections.The volume density of CD34 positive endothelial cells and surrounded capillary lumen in glomeruli was measured using a combination of immunohistochemical staining and the stereological grid system.Results The capG was divided into early,middle,and late phases,and middle phase capG was subdivided into early-middle and late-middle phases,according to the morphology of developing glomeruli and the arrangement of podocytes.As result,the volume density of capillary loops in early phase capG could not be measured due to the complex"glomerular"shape.The volume density of capillary loops increased from(35.95±6.45)% in the early-middle phase capG,to(58.36±6.30)% in the late-middle phase capG,and to(79.89±5.21)% in the late phase capG,compared to(93.61±1.96)% in the mature glomerulus.Furthermore,the volume density of capillary loops remained constant at same stage even though at different developmental time points.Conclusion This study demonstrated a significantly increased volume density of capillary loops with the kidney development.In addition,the results provide a descriptive and reliable parameter for the evaluation of glomerular development.
关 键 词:肾脏 发育 CD34 毛细血管袢期肾小球 形态计量学 免疫组织化学 小鼠
分 类 号:R329.1[医药卫生—人体解剖和组织胚胎学]
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.179