DDAB/Tween 80阳离子脂质体的构建与评价  

Construction and evaluation of DDAB/Tween 80 cationic liposome

作　　者：乔华[1,2,3] Ajab Khan 张慧超 QIAO Hua;AJAB Khan;ZHANG Hui-chao(Shanxi Key Laboratory of Birth Defect and Cell Regeneration,Shanxi Medical University,Taiyuan 030001,China;Key Laboratory of Cellular Physiology(Shanxi Medical University),Ministry of Education,Taiyuan 030001,China;School of Basic Medical Sciences,Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学出生缺陷与细胞再生山西省重点实验室,山西太原030001 [2]山西医科大学细胞生理学教育部重点实验室,山西太原030001 [3]山西医科大学基础医学院,山西太原030001

出　　处：《化学研究与应用》2021年第10期1918-1925,共8页Chemical Research and Application

基　　金：山西省青年科技研究基金项目(201901D211339)资助;山西省重点学科建设经费项目资助。

摘　　要：阳离子脂质体是基因转染中最受关注的一种非病毒类载体。本文以双十二烷基二甲基溴化铵(DDAB)与聚山梨酯-80(Tween 80)复配构建阳离子脂质体,并评价其使用安全性和DNA压缩性能。结果显示,DDAB与Tween 805∶5复配形成的脂质体,粒径和Zeta电位适中,对溴酚蓝产生较强的包封作用;同时,可以有效地抑制DDAB的细胞毒性,在≤800μM时,红细胞溶血率<5%,表现出优良的使用安全性;当该脂质体与DNA质量比为4时,对DNA显示出较强地结合压缩作用,且形成的复合物带有较高的正电荷(约+40 mV),这为基因转染提供了良好的理论基础。Cationic liposome is one of the most crucial non-viral vectors in gene transfection.In this study,a liposome was constructed by the combination of didecyl dimethyl ammonium bromide(DDAB)and polysorbate-80(Tween 80),and its biosafety and DNA compression performance were evaluated.The results showed that the liposome was constructed by mixing DDAB and Tween 80 with the ratio of 5∶5 possessed moderate particle size and zeta potential,which could produce strong entrapment effect on bromophenols.Moreover,at concentration less than 800μM,the liposome possessed excellent biosafety level by having less than 5%hemolysis rate and effectively inhibited the cytotoxicity of DDAB.When the mass ratio of the liposome to DNA was 4,it produced strong compression on DNA and the complex formed had a relatively high positive charge(about+40 mV)which provided a good theoretical basis for gene transfection.

关 键 词：阳离子脂质体 DDAB/Tween 80复配体系 细胞毒性 红细胞溶血 DNA压缩性能 

分 类 号：O69[理学—化学]