一种分子荧光光谱法测定铁离子的实验改进  

Experimental Teaching Design for Fe^(3+)-detection in Wastewater Using Molecular Fluorescence Method

作　　者：边永军 渠星宇 何婧[1] 李建晴[1] 陈勇强 BIAN Yong-jun;QU Xing-yu;HE Jing;LI Jian-qing;CHEN Yong-qiang(College of Chemistry and Chemical Engineering,Jinzhong University,Shanxi Jinzhong 030619,China)

机构地区：[1]晋中学院化学化工系,山西晋中030619

出　　处：《广州化工》2021年第20期101-103,共3页GuangZhou Chemical Industry

基　　金：山西省高等学校教学改革创新项目(J2020317、J2021656);晋中学院教学改革创新项目(Jg202039)。

摘　　要：改进了一个大学化学实验——采用分子荧光光谱法测定铁离子的含量。该实验选用了一个BODIPY(硼氟二吡咯亚甲基)类荧光探针替换教材实验中8-羟基喹啉探针,操作简单,能够快速、高效、高选择性的检测铁离子。采用标准曲线法,有效的把探针和铁离子浓度建立线性关系,从而计算得到废水中铁离子含量。通过本实验可以使大学生快速掌握分子荧光法的基础知识,能够锻炼大学生运用所学实验技术解决实际问题的能力,适合作为本科生仪器分析实验项目开设。An university Chemistry experiment on the detection of iron ions using a molecular fluorescence method was improved.In the experiment,the traditional 8-hydroxyquinoline was replaced by a compound based on BODIPY,which was simple in operation and high in efficiency and selectivity.The standard curve method was used to effectively establish a linear relationship between the probe and the iron ion concentration,thereby calculating the iron ion content in the wastewater.Through this experiment,college students can not only quickly master the basic knowledge of molecular fluorescence method,but also exercise the ability of college students to use the experimental techniques to solve practical problems.This experiment is suitable for senior Instrumental Analysis experiments.

关 键 词：分子荧光法 铁离子 BODIPY类探针 荧光探针 

分 类 号：O652.1[理学—分析化学]