明胶-柠檬酸-硬脂酸复合凝胶的制备及性能  

Preparation and Properties of Gelatin-citric Acid-stearic Acid Composite Gel

作　　者：郭华 史泽毅 张海霞 邓博 王亚雄 靳利娥[2] GUO Hua;SHI Ze-yi;ZHANG Hai-xia;DENG Bo;WANG Ya-xiong;JIN Li-e(Office of Academic Research,Taiyuan University of Technology,Taiyuan 030024,China;College of Chemistry and Chemical Engineering,Taiyuan University of Technology,Taiyuan 030024,China)

机构地区：[1]太原理工大学科学技术研究院,山西太原030024 [2]太原理工大学化学化工学院,山西太原030024

出　　处：《现代食品科技》2021年第10期171-179,307,共10页Modern Food Science and Technology

基　　金：国家自然科学基金项目(51174144);山西省高等学校科技成果转化培育项目(201930)。

摘　　要：针对明胶分子链刚性差,单一组分制备的凝胶机械性能和保水性较差,降解速率快的问题,该研究以明胶为原料,柠檬酸为交联剂,利用疏水性物质硬脂酸对其进行改性,制备明胶-柠檬酸-硬脂酸(Gelatin-Citric Acid-Stearic Acid,GA-CA-SA)复合凝胶。以压缩强度为响应值优化了明胶-硬脂酸复合凝胶的制备工艺条件,利用扫描电子显微镜观察其形貌、红外光谱仪对化学结构进行了表征,并对其进行了细胞毒性评价和姜黄素缓释研究。结果表明:最佳工艺参数为硬脂酸浓度0.013 g/L,柠檬酸浓度0.015 g/mL,温度69℃,在此条件下制备的GA-CA-SA复合凝胶压缩强度为99.05 kPa,较明胶(Gelatin,GA)凝胶压缩强度(45.55 kPa)来说,力学强度提高了117%。红外光谱表明,由于柠檬酸的交联作用,改变了明胶的二级结构,使得形成的GA-CA-SA复合凝胶,亲水性的明胶排列在外面,疏水性的硬脂酸包裹在里边,形成了规则的孔隙结构。且无细胞毒性,对姜黄素具有较好的缓慢释放的效果。In order to enhance the mechanical properties of gelatin gel and expand its application value,gelatin-citric acid-stearic acid composite gel was prepared by using gelatin as raw material,citric acid as cross-linking agent and stearic acid as additive.The preparation conditions of composite gel were optimized with compressive strength as the response value.The morphology of the composite was observed with a scanning electron microscope(SEM),the chemical structure was characterized by infrared spectroscopy(FT-IR),and the cytotoxicity and curcumin release were studied.The results showed that the optimized process conditions were as follows:the quality of stearic acid was 0.013 g/mL,the quality of citric acid was 0.015 g/mL,and the temperature was 69℃.The mechanical strength of the gelatin-stearic acid composite gel(99.05 kPa)prepared under the optimal conditions was 117%higher than that of the single component gelatin gel(45.55 kPa).The FT-IR spectrum analysis showed that the secondary structure of gelatin was changed due to the cross-linking effect of citric acid,resulting in the formation of GA-CA-SA composite gel.The hydrophilic gelatin was arranged outside and the hydrophobic stearic acid was wrapped in it.Inside,a regular pore structure was formed.It has no cytotoxicity and has a good slow release effect on curcumin.

关 键 词：明胶 硬脂酸 柠檬酸 复合凝胶 力学强度 

分 类 号：TQ427.26[化学工程]