金霉素和乳果糖-凝结芽孢杆菌合生素对断奶仔猪相对生长速率和粪便代谢产物的影响  

Effects of Chlortetracycline and Synbiotic with Lactulose and Bacillus coagulans on Relative Growth Rate and Fecal Metabolites of Weaned Piglets

作　　者：杨运南 赵祖艳 刘日亮 张云龙 胡浩 郑卫江[1] 姚文[1] YANG Yunnan;ZHAO Zuyan;LIU Riliang;ZHANG Yunlong;HU Hao;ZHENG Weijiang;YAO Wen(Jiangsu Key Laboratory of Gastrointestinal Nutrition and Health,Laboratory of Gastrointestinal Microbiology,Nanjing Agricultural University,Nanjing 210095,China;Jiangsu Yuanshan Biological Technology Co.,Ltd.,Yancheng 224001,China)

机构地区：[1]南京农业大学消化道生物实验室,江苏省消化道营养与动物健康重点实验室,南京210095 [2]江苏远山生物技术有限公司,盐城224001

出　　处：《动物营养学报》2021年第10期5534-5544,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家重点研发计划(2017YFE0114400);江苏现代农业(生猪)产业技术体系营养调控岗位项目(JATS[2020]412)。

摘　　要：本试验旨在研究金霉素和乳果糖-凝结芽孢杆菌合生素对断奶仔猪相对生长速率及粪便代谢产物的影响。选取18头健康且体重相近的27~28日龄“杜×长×大”阉公猪,初始体重(9.08±0.59)kg,随机分为3组,每组6个重复,每个重复1头猪。对照组(CTR组)饲喂基础无抗饲粮,抗生素组(ANT组)饲喂基础无抗饲粮+75 mg/kg金霉素,合生素组(SYN组)饲喂基础无抗饲粮+乳果糖-凝结芽孢杆菌合生素(10 g/kg乳果糖+2×10^(9) CFU/kg凝结芽孢杆菌)。试验期为29 d。试验期间每周记录体重,并对第29天粪便样品进行代谢组学与短链脂肪酸(SCFAs)检测。结果表明:1)第1~4周,3组相对生长速率(RGR)存在显著的趋势(P=0.052);其中SYN组比CTR组高6.00%,ANT组比CTR组高7.08%,而ANT组比SYN组高1.01%。2)粪便代谢组学结果显示,与CTR组相比,ANT组和SYN组均能显著影响有机酸和胺类物质的含量(P<0.05);其中,除L-哌啶甲酸和吡多胺含量均显著提高(P<0.05)外,无其他共有差异代谢产物。与ANT组相比,SYN组亚油酸、反异油酸、所有顺式(6,9,12)-亚油酸、D-乳糖、环己胺、烟酸、腺嘌呤和N6,N6,N6-三甲基-L-赖氨酸含量显著提高(P<0.05),5-氨基戊酸、D-半乳糖醛酸和2-氧己二酸含量显著降低(P<0.05)。3)KEGG通路富集结果显示,与CTR组相比,SYN组和ANT组均显著影响赖氨酸降解通路(P<0.05);此外,ANT组还显著影响苯丙氨酸代谢、维生素消化吸收、精氨酸和脯氨酸代谢3条通路(P<0.05);SYN组则影响半乳糖代谢、味觉转导、碳水化合物消化吸收、ABC转运蛋白、色氨酸代谢、淀粉和蔗糖代谢共6条通路(P<0.05)。与ANT组相比,SYN组显著影响赖氨酸降解、亚油酸代谢、ABC转运蛋白和不饱和脂肪酸生物合成共4条通路(P<0.05)。4)3组之间SCFAs浓度无显著差异(P>0.05),但SYN组丙酸比例显著低于ANT组(P<0.05)。5)相关性分析结果显示,断奶仔猪RGR与粪便代谢产物D-半乳糖醛酸、环己胺和甘This experiment aimed to study the effects of synbiotic with lactulose and Bacillus coagulans on relative growth rate and fecal metabolites of weaned piglets. Eighteen 27 to 28 days of age Duroc×Landrace×Yorkshire weaned castrated boars with similar body weight of(9.08±0.59)kg were selected and randomly divided into 3 groups with 6 replicates in each group and 1 pig in each replicate. The control group(CTR group)was fed an antibiotic-free basal diet,the antibiotic group(ANT group)was fed an antibiotic-free basal diet +75 mg/kg chlortetracycline,and the synbiotic group(SYN group)was fed an antibiotic-free basal diet+synbiotic with lactulose and Bacillus coagulans(10 g/kg laculose+2×10^(9) CFU/kg Bacillus coagulans). The experiment lasted for 29 days. During the experiment,the body weight was recorded every week,and the fecal samples were collected on day 29 for metabolomics and short chain fatty acids(SCFAs)analysis. The results showed as follows:1)during 1 to 4 weeks,a significant tendency was detected in RGR in 3 groups(P =0.052). Compared with CTR group,the RGR in SYN group was increased by 6.00%,while the RGR in ANT group was increased by 7.08%;and the RGR in ANT group was 1.01% greater than SYN group. 2)The fecal metabolomics results showed that compared with CTR group,both ANT group and SYN group could significantly affect the content of organic acids and amines;among them,except the content of L-pipecolic acid and pyridoxine were significant increased(P<0.05),there were no other differential metabolites. Compared with the ANT group,the contents of linoleic acid,trans-vaccenic acid,all cis-(6,9,12)linoleic acid,D-lactose,cyclohexylamine,nicotinate,adenine,and N6,N6,N6-trimethyl-L-lysine in SYN group were significantly increase(P<0.05),and the contents of 5-aminovaleric acid,D-galacturonic acid,and 2-oxoadipate were significantly decrease(P< 0.05). 3)The KEGG pathway enrichment results showed that compared with CTR group,both SYN group and ANT group significantly affected the lysine degradation pathway(P<

关 键 词：乳果糖 凝结芽孢杆菌 合生素 断奶仔猪 生长速率 粪便 

分 类 号：S828[农业科学—畜牧学]