植物炭黑对采食玉米赤霉烯酮污染饲粮的大鼠生长性能和生殖系统的影响  

Effects of Vegetable Carbon Black on Growth Performance and Reproductive System of Rats Fed Zearalenone Contaminated Diet

作　　者：刘淑杰[1] 邓波[1] 吴杰[1] 徐子伟[1] 马倩倩 LIU Shujie;DENG Bo;WU Jie;XU Ziwei;MA Qianqian(Institute of Animal Husbandry and Veterinary Science,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]浙江省农业科学院畜牧兽医研究所,杭州310021

出　　处：《动物营养学报》2021年第10期5827-5837,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：浙江省重点研发计划项目(2021C02007);浙江省科技厅资助项目(2016C02054);国家现代农业产业技术体系建设专项资金资助(CARS-36);国家青年科学基金项目(C170105/31501966)。

摘　　要：本文通过研究在玉米赤霉烯酮(ZEN)污染的饲粮中添加不同剂量的植物炭黑对大鼠生长性能和生殖系统的影响,旨在评价植物炭黑对ZEN的吸附效果和对大鼠健康的改善作用,为其用于畜牧生产及饲料行业提供依据。试验采用完全随机设计,选用64只体重约为150 g的5~6周龄无特定病原体(SPF)级雌性SD大鼠,随机分为4个组,每组16个重复,每个重复1只。4个组分别为:1)空白对照组,饲喂基础饲粮;2)负对照组,饲喂基础饲粮+25 mg/kg ZEN;3)低剂量植物炭黑组,饲喂基础饲粮+25 mg/kg ZEN+1.0 g/kg植物炭黑;4)高剂量植物炭黑组,饲喂基础饲粮+25 mg/kg ZEN+1.5 g/kg植物炭黑。试验预试期7 d,正试期28 d。结果表明:1)与空白对照组相比,负对照组大鼠第1~14天平均日增重(ADG)显著降低(P<0.05),第1~28天ADG极显著降低(P<0.01),分别降低了26.06%和28.45%;负对照组大鼠第1~14天料重比(F/G)显著升高(P<0.05),第15~28天和第1~28天F/G极显著升高(P<0.01),分别升高了33.08%、28.21%和40.73%。与负对照组相比,高剂量植物炭黑组大鼠第15~28天和第1~28天F/G显著降低(P<0.05),分别降低了15.90%和20.89%。2)与空白对照组相比,负对照组大鼠第14和28天血清碱性磷酸酶(ALP)活性极显著升高(P<0.01),第28天血清谷丙转氨酶(GPT)活性显著升高(P<0.05),第28天血清谷草转氨酶(GOT)活性极显著升高(P<0.01),分别升高了37.67%、44.17%、42.55%和37.75%。与负对照组相比,高剂量植物炭黑组大鼠第14天血清ALP活性极显著降低(P<0.01),第28天血清GPT和GOT活性显著降低(P<0.05),分别降低了22.48%、24.48%和20.27%。3)与空白对照组相比,负对照组大鼠第14天血清促卵泡素(FSH)浓度极显著升高(P<0.01),第28天血清FSH浓度显著升高(P<0.05),分别升高了20.40%和19.86%;负对照组大鼠第14和28天血清雌二醇(E2)浓度极显著降低(P<0.01),分别降低了19.30%和17.59%;负对照组大鼠第14天血清促黄体素(LH)浓度极显著降�In this study,the effects of different doses of dietary vegetable carbon black in the zearalenone(ZEN)contaminated diet on the growth performance and reproductive system of rats were studied,so as to evaluate the adsorption effects of vegetable carbon black on ZEN and the improvement effects on the health of rats,and to provide a basis for its use in animal husbandry and feed industry.In a completely randomized design,sixty-four 5-to 6-week-old specific pathogen free(SPF)female SD rats with a body weight of 150 g were randomly divided into 4 groups with 16 replicates per group and 1 rat per replicate.The four groups were as follows:1)the blank control group,and the rats were fed a basal diet;2)the negative control group,and the rats were fed the basal diet+25 mg/kg ZEN;3)the low-dose vegetable carbon black group,and the rats were fed the basal diet+25 mg/kg ZEN+1.0 g/kg vegetable carbon black;4)the high-dose vegetable carbon black group,and the rats were fed the basal diet+25 mg/kg ZEN+1.5 g/kg vegetable carbon black.The experimental period lasted for 28 days after 7-day adaption.The results showed as follows:1)compared with the blank control group,the average daily gain(ADG)of rats in the negative control group was significantly decreased by 26.06%from days 1 to 14(P<0.05),and was extremely significantly decreased by 28.45%from days 1 to 28(P<0.01);the ratio of feed to gain(F/G)in the negative control group was significantly increased by 33.08%from days 1 to 14(P<0.05),and was extremely significantly increased by 28.21%and 40.73%from days 15 to 28 and days 1 to 28(P<0.01),respectively.Compared with the negative control group,the F/G of rats in the high-dose vegetable carbon black group was significantly decreased by 15.90%and 20.89%from days 15 to 28 and days 1 to 28(P<0.05),respectively.2)Compared with the blank control group,the serum alkaline phosphatase(ALP)activity of rats in the negative control group was extremely significantly increased by 37.67%and 44.17%on days 14 and 28(P<0.01),the serum glutamic-pyru

关 键 词：玉米赤霉烯酮 植物炭黑 吸附剂 大鼠 生长性能 生殖系统 

分 类 号：S816[农业科学—饲料科学]