红参提取物通过抑制凋亡减轻H2O_(2)诱导大鼠心肌细胞氧化损伤作用研究  

Effects of Rge on H2O_(2)-induced oxidative damage of rat cardiomyocytes via inhibiting apoptosis

作　　者：张雨曦 庄倩 李玉倩 石林 章卓 姜鲜 Zhang Yu-xi;Zhuang Qian;Li Yu-qian;Shi Lin;Zhang Zhuo;Jiang Xian(Graduate Student 2019,School of Pharmacy,Southwest Medical Unive rsity,Luzhou 646000,Sichuan,China;Depa rtment of Pharmacology,Southwest Medical University,Luzhou 646000,Sichuan,China;Department of Anesthesiology,Luzhou People's Hospital,Luzhou 646000,Sichuan,China)

机构地区：[1]西南医科大学药学院,四川泸州646000 [2]西南医科大学药学院药理教研室,四川泸州646000 [3]泸州市人民医院麻醉科,四川泸州646000

出　　处：《四川生理科学杂志》2021年第8期1289-1292,1388,共5页Sichuan Journal of Physiological Sciences

基　　金：国家级创新创业训练项目(编号:201810632061)。

摘　　要：目的:探讨红参提取物(Red ginseng extract,Rge)对过氧化氢(Hydrogen peroxide,H2O_(2))诱导H9c2心肌细胞氧化损伤的保护作用及机制.方法:体外培养H9c2心肌细胞并随机分为5组,即对照组,模型组,Rge低、中、高浓度组.对照组常规培养,模型组以9 mg·kg^(-1)的H2O_(2)处理6 h建立体外细胞氧化损伤模型,而Rge低、中、高浓度组造模前以200、400、800 mg·kg^(-1)Rge预处理细胞24 h.采用MTT法检测细胞活力;化学法检测LDH释放水平;JC-1染色法检测线粒体膜电位;Western blot法检测Caspase-3、Bax、Bcl-2的相对表达水平.结果:与模型组相比,Rge各浓度组H9c2细胞活力均明显增加(P<0.05),LDH释放水平均明显降低(P<0.001),且膜电位呈现剂量依赖性增高,Caspase-3、Bax表达水平下调且Bcl-2表达水平上调(P<0.001).结论:Rge可能通过降低LDH的释放和抑制凋亡相关蛋白的表达,提高H9c2心肌细胞对抗H2O_(2)氧化损伤的能力.Objective:To investigate the protective effect and mechanism of Red ginseng extract(Rge)on hydrogen peroxide(H2O_(2))induced oxidative damage of H9c2 cardiomyocytes.Methods:H9c2 cardiomyocytes were cultured and randomly divided into 5 groups:control group,model group and low,medium and high concentration groups of Rge.The control group was cultured routinely.The model group was treated with 9 mg·kg^(-1) H2O_(2) for 6 hours to establish the cell oxidative damage model in vitro.The cells in the low,medium and high concentration groups were pretreated with 200,400 and 800 mg·kg^(-1) Rge for 24 hours,and then treated with 9 mg·kg^(-1) H2O_(2) for 6 hours.MTT assay was used to detect cell viability;LDH release level was detected by chemical method;mitochondrial membrane potential was detected by JC-1 staining and levels of Caspase-3,Bax and Bcl-2 were detected by Western blot.Results:Compared with the model group,the viability of H9c2 myocardial cells in each concentration group of Rge were significantly increased(P<0.05),the LDH release level was significantly reduced(P<0.001),and the membrane potential increased in a dose-dependent manner,Caspase-3 and Bax levels were down-regulated and Bcl-2 level was up-regulated(P<0.001).Conclusion:Rge may protect H9c2 cardiomyocytes from oxidative damage by H2O_(2) by reducing the release of LDH and inhibiting the expression of apoptosis related proteins.

关 键 词：红参提取物 心肌细胞 乳酸脱氢酶 凋亡 

分 类 号：R28[医药卫生—中药学]