蛇伤胶囊中5种蒽醌类化合物含量的测定  

DETERMINATION OF FIVE ANTHRAQUINONES IN SHESHANG CAPSULES

作　　者：张华[1] 于虹敏[1] 严国鸿[2] 孙承韬 ZHANG hua;YU Hong-min;YAN Guo-hong;SUN Cheng-tao(Pharmacy College,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China;People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350004,China)

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]福建中医药大学附属人民医院,福建福州350004

出　　处：《井冈山大学学报（自然科学版）》2021年第5期98-101,共4页Journal of Jinggangshan University (Natural Science)

基　　金：福建省科技厅科技引导性项目(2017Y0047)。

摘　　要：目的建立蛇伤胶囊中5种蒽醌类成分含量测定分析方法,提升蛇伤胶囊质量标准。方法采用Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇(A)-0.1%磷酸水溶液(B),梯度洗脱(0~7 min,75%A;7~30 min,75%A→90%A;30~40 min,90%A),流速为1.0 mL·min^(-1),检测波长为254 nm,进样量10μL。结果5个待测组的线性范围:4.04~40.40μg/mL(r=0.9994)、4.16~41.60μg/mL(r=0.9991)、4.14~41.40μg/mL(r=0.9959)、4.10~41.00μg/mL(r=0.9993)和2.16~21.60μg/mL(r=0.9997)。平均回收率(n=6)分别为100.02%、100.02%、100.03%、99.99%和100.06%,RSD分别为0.40%、0.15%、0.30%、0.11%和0.47%。结论所建立的方法操作简便准确且具有良好的重复性,可用于测定蛇伤胶囊中芦荟大黄素、大黄素、大黄酸、大黄酚和大黄素甲醚的含量。Objective:To establish a quantitative determination method of HPLC for five anthraquinones and improve the quality control standard of Sheshang capsules.Methods:The separation was performed on a Diamonsil-C_(18) column(250 mm×4.6 mm,5μm)with a gradient elution(0-7 min,75%A;7-30 min,75%A→90%A;30~40 min,90%A)of the mobile phase composed of methanol(A)and 0.1%phosphoric acid(B).The flow rate was 1.0 mL.min^(-1) and the detection wavelength was 254 nm.The injection volume was 10μL.Results:The linear ranges of five anthraquinones were 4.04~40.40μg/mL(r=0.9994),4.16~41.60μg/mL(r=0.9991),4.14~41.40μg/mL(r=0.9959),4.10~41.00μg/mL(r=0.9993)and 2.16~21.60μg/mL(r=0.9997),respectively,and their average recoveries respectively were 100.02%,100.02%,100.03%,99.99%and 100.06%,RSD respectively were 0.40%,0.15%,0.30%,0.11%and 0.47%(n=6).Conclusion:The established method is simple,accurate and reproducible,can be used to determine the contents of aloe emodin,emodin,rhubarb,rhubarb phenol and emodin methy in Sheshang capsules.

关 键 词：高效液相色谱法 蛇伤胶囊 蒽醌类 含量测定 

分 类 号：R284.1[医药卫生—中药学]