普通小麦颖壳蜡质缺失突变体glossy1的转录组分析  

Transcriptome profiling of glossy1 mutant with glossy glume in common wheat(Triticum aestivum L.)

作　　者：李玲红 张哲 陈永明 尤明山[1] 倪中福[1] 邢界文 LI Ling-Hong;ZHANG Zhe;CHEN Yong-Ming;YOU Ming-Shan;NI Zhong-Fu;XING Jie-Wen(College of Agronomy,China Agricultural University,Beijing 100193,China)

机构地区：[1]中国农业大学农学院,北京100193

出　　处：《作物学报》2022年第1期48-62,共15页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31991214);国家重点研发计划项目(2017YFD0101004)资助。

摘　　要：为了明确突变体颖壳蜡质含量显著变化的分子机制,本研究对源自济麦22颖壳蜡质缺失突变体glossy1与野生型进行了转录组分析。结果表明,在glossy1突变体中,共筛选到12,230个差异表达基因,其中5811个基因在突变体中上调表达,6419个下调表达。GO(gene ontology)功能富集分析发现,差异基因主要富集在蜡质合成和转运途径,具体分布在酰基转移酶活性、脂质结合和水解酶活性等条目,由此推测这些途径与小麦穗部蜡质缺失性状是紧密相关的。我们还利用RT-qPCR检测了参与蜡质代谢途径部分基因的表达,结果与转录组结果是一致的。本研究为今后探究小麦蜡质代谢的分子机制和基因调控网络提供了数据支持,同时也为抗逆小麦育种奠定了理论基础。In order to clarify the molecular mechanism of the significant change in glume wax content,transcriptome profiling was performed on glossy glume mutant glossy1 and its wild-type Jimai 22.The results showed that a total of 12,230 differentially expressed genes were screened in the glossy1 mutant,among which 5811 genes were up-regulated and 6419 were down-regulated.GO functional enrichment revealed that the differentially expressed genes were mainly enriched in the wax synthesis and transport pathway,specifically distributed in acyltransferase activity,lipid binding,hydrolase activity.The findings suggested that these pathways were closely related to the glume-wax-deficient trait in wheat.We also detected the relative expression levels of some genes involved in the wax metabolic pathway by RT-qPCR,and the results were consistent with the transcriptome data.This study not only provides data support for the further study of the molecular mechanism of wax metabolism and gene regulation network but also lays a theoretical foundation for the breeding of stress-resistant variety in wheat.

关 键 词：小麦 表皮蜡质 glossy1突变体 转录组分析 分子机制 

分 类 号：S512.1[农业科学—作物学]