基于网络药理学的黄芪免疫调节机制  

Exploration on immunoregulation mechanism of Astragalus based on network pharmacology

作　　者：赵珈华 刘洋[1] 桂金秋[1] 石学魁[1] 唐小云[1] ZHAO Jia-hua(Department of Pathogenic Biology,Mudanjiang Medical University,Mudanjiang 157011,China)

机构地区：[1]牡丹江医学院病原生物学教研室,黑龙江牡丹江157011

出　　处：《牡丹江医学院学报》2021年第5期13-18,共6页Journal of Mudanjiang Medical University

基　　金：黑龙江省卫生健康委科研课题项目(2019-390)。

摘　　要：目的采用网络药理学的方法研究黄芪的主要活性成分、靶点,初步探讨其免疫调节的作用机制。方法通过中药系统药理学数据库(TCMSP)搜索黄芪活性成分和成分对应作用靶点。通过GeneCards数据库筛选免疫调节相关的靶点信息。采用RStudio软件将黄芪的药物活性靶点与免疫调节靶点信息取交集,将交集靶点数据依靠String在线数据库平台构建蛋白互作PPI网络模型,利用Cytoscape 3.7.2软件,构建可视化的“中药-成分-靶点-疾病”网络图并进行分析,找寻黄芪免疫调节的关键靶点并通过RStudio绘制柱状图。使用RStudio对关键靶点进行GO功能分析和KEGG通路分析。结果黄芪免疫调节的潜在靶点有42个,PPI网络模型提示TP53、AKT-1、IL-6、TNF等可能是黄芪免疫调节的核心靶点。GO功能分析结果显示,黄芪免疫调节潜在基因的生物功能主要涉及细胞因子受体结合、细胞因子活性、信号受体激活剂活性、生长因子受体结合、参与凋亡信号通路的半胱氨酸型内肽酶活性等;KEGG通路分析结果显示,黄芪免疫调节潜在基因的通路主要涉及糖尿病并发症中的AGE-RAGE信号通路(AGE-RAGE signaling pathway in diabetic complications)、疟疾(Malaria)、乙型肝炎(Hepatitis B)、查加斯病(Chagas disease)等信号通路。结论本研究通过网络药理学分析表明中药黄芪通过多个靶点调控多条信号转导通路发挥免疫调节作用,为黄芪的进一步研究和应用奠定了基础。Objective To study the main active components and targets of Astragalus by means of network pharmacology, and to preliminarily explore the mechanism of its immune regulation.Methods The active ingredients of Astragalus and its corresponding targets were searched through the Traditional Chinese Medicine System Pharmacology Database(TCSP).The target information related to immune regulation was screened by GeneCards database.RStudio software was used to collect the information of drug active targets and immune regulatory targets of Astragalus.The PPI network model of protein interaction relationship was constructed by String online database platform.Using the software of Cytoscape3.7.2,the visualized network diagram of "Chinese Medicine-components-targets-diseases" was constructed and analyzed to find the key targets of Astragalus immune regulation, and the histogram was drawn by RStudio software.Rstudio software was used to analyze the GO function and KEGG pathway of key targets.Results There were 42 potential targets of Astragalus immunoregulation.The PPI network model suggests that TP53,AKT-1,IL-6 and TNF,etc.may be the core targets of Astragalus immunoregulation.GO functional analysis showed that the biological functions of potential immunoregulation genes of Astragalus mainly involved cytokine receptor binding, cytokine activity, receptor ligand activity, signal receptor activator activity, growth factor receptor binding, growth factor activity, and semi-Cystine endopeptidase activity involved in apoptosis signaling pathway, etc.KEGG pathway analysis showed that the potential gene pathways of Astragalus were mainly involved in AGE-RAGE signaling pathway in diabetic complications, malaria, hepatitis B,Chagas disease and other signaling pathways.Conclusion In this study, we found that, by the network pharmacology analysis, Astragalus played the role of immune regulation through regulating multiple signal transduction pathways through multiple targets which laid a foundation for further research and application of

关 键 词：黄芪 免疫调节 活性成分 网络药理学 靶点 信号通路 

分 类 号：R285.5[医药卫生—中药学] R392.5[医药卫生—中医学]