电刺激对背根神经节和雪旺细胞髓鞘化的影响  

Effects of electrical stimulation on the myelination of dorsal root ganglia and Schwann cells

作　　者：雷涛 刘玉红 张煜 梁卓文 LEI Tao;LIU Yuhong;ZHANG Yu;LIANG Zhuowen(Department of Biomedical Engineering,Air Force Medical University,Xi'an 710032,China;Department of Stomatology,Stomatological Hospital,Air Force Medical University,Xi'an 710032,China;The 901st Hospital of People's Liberation Army Joint Logistic Support Force,Hefei 230031,China;Xijing Hospital,Air Force Medical University,Xi'an 710032,China)

机构地区：[1]空军军医大学生物医学工程系,陕西西安710032 [2]空军军医大学口腔医院口腔科,陕西西安710032 [3]联勤保障部队解放军第901医院,安徽合肥230031 [4]空军军医大学西京医院,陕西西安710032

出　　处：《中国医学物理学杂志》2021年第10期1285-1288,共4页Chinese Journal of Medical Physics

基　　金：国家自然科学基金(51907197)。

摘　　要：目的:建立体外的背根神经节(DRG)—雪旺细胞(SCs)—电刺激模型,为研究电刺激促周围神经成髓鞘机制提供基础。方法:以函数发生器和电刺激小室构建电刺激细胞培养系统,DRG/SCs共培养24 h后分为对照(Ctrl)组和电刺激(ES)组。ES组给予矩形波电刺激,6 Vp-p,10 Hz,1 h/d,7 d。Ctrl组无电刺激。利用CCK8观测电刺激对细胞毒性的影响,利用髓磷脂碱性蛋白(MBP)免疫荧光检测电刺激对DRG/SCs髓鞘化的影响。结果:CCK8细胞增殖毒性实验显示,ES组光密度值略高于Ctrl组,但两组之间无统计学意义。免疫荧光检测结果显示ES组MBP的荧光强度明显高于Ctrl组(P<0.01)。结论:本文设计的电刺激细胞培养系统对神经细胞安全无毒,连续7 d的电刺激可提高神经髓鞘化率。Objective To establish an in vitro dorsal root ganglion(DRG)-Schwann cells(SCs)-electrical stimulation(ES)model for providing a basis for the investigation on the mechanism of ES in promoting peripheral nerve myelination.Methods A function generator and an ES chamber were used to construct an ES cell culture system.After being co-cultured for 24 h,DRG/SCs were divided into control group and ES group.The ES group was given rectangular wave ES,6 Vp-p,10 Hz,1 h/d,for 7 days;and no ES was applied in control group.The effect of ES on cytotoxicity was observed using CCK8,and the effect of ES on the myelination of DRG/SCs was detected by immunofluorescence using myelin basic protein.Results The CCK8 cell proliferation and toxicity assay showed that the optical density of ES group was slightly higher than that of control group,but there was no statistical significance between two groups.The immunofluorescence assay revealed that the fluorescence intensity of myelin basic protein in ES group was significantly higher than that in control group(P<0.01).Conclusion The ES cell culture system designed in the study is safe and non-toxic to neuronal cells,and ES for 7 days in succession can increase the rate of neural myelination.

关 键 词：电刺激 导电玻璃 背根神经节 雪旺细胞 髓鞘化 

分 类 号：R318[医药卫生—生物医学工程]