中国特有濒危植物八角莲胚状体的发生及其植株再生研究  

Ecological Occurrence and Plant Regeneration of Embryoid of the Endangered and Endemic Plant Dysosma versipellis in China

作　　者：谭小明 周雅琴[1,3] 陆玫霖[1,2] 傅鹏 唐红珍[1] TAN Xiaoming;ZHOU Yaqin;LU Meilin;FU Peng;TANG Hongzhen(Guangxi University of Chinese Medicine,Nanning,Guangxi 530200;Guangxi Engineering Technology Research Center of Advantage Chinese Patent Drug and Ethnic Drug Development,Nanning,Guangxi 530200;Guangxi Botanical Garden of Medicinal Plant,Nanning,Guangxi 530023)

机构地区：[1]广西中医药大学,广西南宁530200 [2]广西优势中成药与民族药开发工程技术中心,广西南宁530200 [3]广西药用植物园,广西南宁530023

出　　处：《北方园艺》2021年第19期121-127,共7页Northern Horticulture

基　　金：国家重点研发计划资助项目(2019YFC1712305);国家自然科学基金资助项目(81360682,31860128);广西一流学科资助项目(2019XK094);2020年广西高校综合实力提升工程资助项目(05020058)。

摘　　要：以我国特有濒危药用植物八角莲(Dysosma versipellis(Hance)M.Cheng)为试材,采用DPS软件正交设计法和SPSS的Duncan′s multiple range test,研究了不同植物激素对其愈伤组织形成、胚状体诱导及植株再生的影响,以期为离体培养条件下快速诱导八角莲愈伤组织、胚状体及其植株的再生,以及将来深入研究真菌诱导子诱导八角莲活性成分鬼臼毒素累积的作用机理、信号调控机制和人工种子制作等提供参考依据。结果表明:八角莲叶和叶柄最适合作为诱导愈伤组织的材料;植物激素对愈伤组织形成的影响效果从大到小依次为2,4-D>TDZ>KT>NAA>2-ip,愈伤组织形成的最佳培养基为MS+2,4-D 1 mg·L^(-1)+NAA 0.05 mg·L^(-1)+TDZ 0.5 mg·L^(-1)+2-ip 1 mg·L^(-1);诱导颗粒状愈伤组织胚状体形成的最佳培养基为MS+6-BA 0.5 mg·L^(-1)+NAA 0.1 mg·L^(-1),诱导率为71.33%;胚状体生根和植株再生培养基为MS+IBA 0.5 mg·L^(-1)+GA30.5 mg·L^(-1)。Taking Dysosma versipellis(Hance)M.Cheng which is an endemic and endangered medicinal plant in China as the test material,the effects of different plant hormones on callus formation,embryoid induction and plant regeneration of Dysosma versipellis(Hance)M.Cheng were studied by orthogonal design of DPS software and Duncan′s multiple range test of SPSS,in order to provide a reference for the rapid induction of callus,embryoid and plant regeneration of D.versipellis under in vitro culture conditions,as well as the in-depth study of the mechanism of action,signal regulation mechanism and artificial seed production of podophyllotoxin accumulation induced by fungal elicitors in D.versipellis.The results showed that leaves and petioles were the most suitable materials in inducing callus.The effect of phytohormone on callus formation followed the order of 2,4-dichlorophenoxyacetic acid(2,4-D)>thidiazuron(TDZ)>kinetin>naphthylacetic acid(NAA)>2-ip.The best medium for callus formation was MS+2,4-D 1 mg·L^(-1)+NAA 0.05 mg·L^(-1)+TDZ 0.5 mg·L^(-1)+2-ip 1 mg·L^(-1).The optimal medium to induce the formation of granular callus embryoid was MS+6-BA 0.5 mg·L^(-1)+NAA 0.1 mg·L^(-1),and the induction rate was 71.33%.The embryoid rooting and plant regeneration medium was MS+IBA 0.5 mg·L^(-1)+GA30.5 mg·L^(-1).

关 键 词：八角莲 愈伤组织诱导 胚状体 植株再生 

分 类 号：R282.71[医药卫生—中药学]