原发性失眠患者中医证型与神经肽S受体基因rs324981多态性的相关性  

On the Correlation between TCM Syndromes and Neuropeptide S Receptor Gene rs324981 Polymorphism in Patients with Primary Insomnia

作　　者：杨军[1] 谢宇平[2] 苏晓艳 马薇[2] 惠培林[2] 周丽雅[2] YANG Jun;XIE Yu-ping;SU Xiao-yan;MA Wei;HUI Pei-lin;ZHOU Li-ya(Gansu University of Traditional Chinese Medicine,Lanzhou Gansu 730000,China;Sleep Medicine Center of People’s Hospital of Gansu Province,Lanzhou Gansu 730000,China)

机构地区：[1]甘肃中医药大学,甘肃兰州730000 [2]甘肃省人民医院睡眠医学中心,甘肃兰州730000

出　　处：《中医药导报》2021年第9期129-132,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金地区科学基金项目(81560228);2018年甘肃省人民医院院内基金项目(8GSSY4-19);2018年甘肃省人民医院院内基金项目(18GSSY4-20)

摘　　要：目的:探讨原发性失眠(PI)患者中医证型与神经肽S受体(NPSR)基因rs324981多态性的相关性。方法:纳入154例PI患者,进行中医辨证分型,并匹配130名健康者为对照组。采用匹兹堡睡眠质量指数量表(PSQI)评估受试者睡眠质量。使用Mass ARRAY SNP基因分型技术检测受试者NPSR基因rs324981多态性,并分析PI患者不同中医证型与NPSR基因rs324981多态性的相关性。结果:154例PI患者中医辨证为肝郁化火证47例(30.52%),痰热内扰证16例(10.39%),阴虚火旺证60例(38.96%),心脾两虚证24例(15.58%),心虚胆怯证7例(4.55%)。PI阴虚火旺证患者NPSR基因rs324981多态性基因型分布与对照组比较,差异有统计学意义[P=0.034,OR=2.10,95%CI(1.05, 4.19)]。PI虚证和实证与对照组NPSR基因rs324981多态性的基因型和等位基因分布比较,差异均无统计学意义(P>0.05)。结论:PI患者阴虚火旺证可能与NPSR基因rs324981多态性存在相关性。Objective:To explore the relationship between TCM syndromes and neuropeptide S receptor(NPSR)gene rs324981 polymorphism in patients with primary insomnia(PI).Methods:Totally 154 PI patients were classified by TCM syndrome differentiation,and 130 healthy persons were matched as the control group.The Pittsburgh Sleep Quality Index(PSQI)was used to evaluate the sleep quality of the subjects.The Mass ARRAY SNP genotyping technology was used to detect the NPSR rs324981 polymorphism of the subjects,and the correlation between NPSR rs324981 polymorphism and TCM syndromes was analyzed in PI patients.Results:According to the dialectic of TCM,154 patients with PI were divided into 47 cases(30.52%)of liver depression transforming into fire syndrome,16 cases(10.39%)of phlegm-heat attacking internally syndrome,60 cases(38.96%)of Yin deficiency fire hyperactivity syndrome,24 cases(15.58%)of heart and spleen deficiency syndrome,and 7 cases(4.55%)of timidity due to heart deficiency syndrome.The genotype distribution of the rs324981 polymorphism of NPSR gene in patients with PI of Yin deficiency fire hyperactivity syndrome was different from that of the control group[P=0.034,OR=2.10,95%CI(1.05,4.19)].There was no significant difference between deficiency and excess syndrome and the control group in the genotype and allele distribution of the NPSR gene rs324981 polymorphism(P>0.05).Conclusion:The Yin deficiency fire hyperactivity syndrome may be related to the rs324981 polymorphism of NPSR gene in PI patients.

关 键 词：原发性失眠 中医辨证 神经肽S受体 基因多态性 

分 类 号：R256.23[医药卫生—中医内科学]