一株新的引起玉米细菌性茎腐病病原细菌的分离鉴定  

Isolation and identification of a new pathogenic bacterium causing bacterial stalk rot of maize

作　　者：姚秀英 代真林 魏兰芳[2] 张晋豪 姬广海[1] YAO Xiu-ying;DAI Zhen-lin;WEI Lan-fang;ZHANG Jin-hao;JI Guang-hai(Ministry of Education Key Laboratory of Agricultural Biodiversity for Plant Disease Management,Yunnan Agricultural University,Kunming 650201,China;Agricultural Foundation Experiment Teaching Center,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学,农业生物多样性与病虫害控制教育部重点实验室,昆明650201 [2]云南农业大学,农科基础实验教学中心,昆明650201

出　　处：《植物病理学报》2021年第5期822-827,共6页Acta Phytopathologica Sinica

基　　金：国家重点研发计划项目(2018YFD0200703);国家重点研发计划项目(2019YFD1002000);云南省"万人计划"产业技术领军人才专项(YNWR-CYJS-2019-046);云南省农业联合重点项目(2017FG001(-005));云南省重点研发(农业领域)计划项目(2018BB016)。

摘　　要：玉米茎腐病是一种在世界玉米产区普遍发生的土传病害,根据病原菌的不同,玉米茎腐病分为细菌性茎腐病和真菌性茎腐病。玉米细菌性茎腐病病原主要有短小芽孢杆菌(Bacillus pumilus Meyer and Gottheil)^([1])、玉米狄克氏菌(Dickeya zeae Samson et al.)^([2])、铜绿假单胞杆菌(Pseudomonas aeruginosa)^([3])、成团泛菌(Pantoea agglomerans)^([4])等。In 2019,the disease was observed in the maize growing areas of Dehong County,Yunnan Province,China.On the basis of signs and symptoms,the disease was characterized as a bacterial infection.Identification of the pathogen were based on morphological characteristics,biology and physiological and biochemical assay,16 S rDNA and specific rpoD and gapA gene sequence analysis.The results show that the pathogen is similar to short rod in shape under a transmission electron microscope,the both ends are obtuse and have 1-3 terminal flagella.In King’s B medium,it produced milky white,round,smooth surface and transparent edge colonies and can utilize the 58 kinds of carbon sources.The LOPAT test result is"+++-+".The construction of evolutionary trees was based on the 16 S rDNA、rpoD and gapA gene sequencing,which indicated the closest genetic relationship with Pseudomonas extremorientalis and which was finally identified as P.extremorientalis.To our acknow-ledgment,this is the first report on the bacterial stalk rot disease of maize caused by phytopathogenic bacteria P.extremorientalis.

关 键 词：玉米茎腐病 玉米细菌性茎腐病 土传病害 玉米产区 铜绿假单胞杆菌 细菌的分离鉴定 

分 类 号：S432.42[农业科学—植物病理学]