蜜环菌发酵液提取物对人参链格孢霉菌的抑制作用  

The Effect of Armillaria mellea Fermentation Broth Extract in Inhibiting Alternaria panax in Ginseng

作　　者：张晨[1] 吕建华 董婧 李长田[1] ZHANG Chen;Lü Jianhua;DONG Jing;LI Changtian(Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学食药用菌教育部工程研究中心,长春130118

出　　处：《菌物研究》2021年第3期177-183,共7页Journal of Fungal Research

基　　金：国家重点研发计划项目(2020YFD1000304)。

摘　　要：为探究蜜环菌Armillaria mellea发酵液提取物的抗植物病原真菌作用,在预实验的基础上选取人参链格孢霉菌Alternaria panax作为供试靶标菌,确定蜜环菌发酵液乙酸乙酯提取物的抑菌效果及其作用机制。结果表明:蜜环菌发酵液乙酸乙酯提取物的MIC值为5 mg/mL,MFC值为20 mg/mL;人参链格孢霉菌孢子萌发抑制率与提取物浓度呈正相关,其孢子萌发EC50值为2.69 mg/mL;显微观察发现,蜜环菌乙酸乙酯提取物处理可明显破坏人参链格孢霉菌菌丝体微观结构。细胞膜通透性和相关生化指标研究结果表明:蜜环菌乙酸乙酯提取物处理后极大提升了人参链格孢霉菌细胞膜通透性,其孢外电导率、孢外核酸含量以及丙二醛(MDA)和过氧化氢(H2O2)含量显著提高,超氧化物歧化酶(SOD)与过氧化物酶(POD)活性在前期略升高后显著降低。据此推测蜜环菌发酵液提取物的抑菌机制为破坏链格孢霉菌菌丝细胞的膜系统,造成菌丝细胞成分的外流并导致胞内生化反应被破坏。In order to study the anti-pathogen effect of the Armillaria mellea fermentation broth extract,on the basis of pretesting,the ethyl acetate extract of the fermentation broth was chosen and the Alternaria panax from ginseng was used as the testing pathogen.The anti-pathogen effect and mechanism of the extract was described.The results showed that MIC and MFC of the ethyl acetate extract was 5 mg/mL and 20 mg/mL respectively.The spore germination EC50 value was 2.69 mg/mL.The ethyl acetate phase extract could significantly destroy the morphology of mycelium cells through microscopic observation.The mycelia components effusion and mycelium biochemical characteristics experiments showed that the conductivity and nucleic acid level outside mycelium cells,the contents of MDA and H2 O2 was significantly increased,and the activity of SOD and POD was slightly increased,and then significantly decreased comparing to the control group.It is deduced that the antifungal mechanism included destroying the membrane system of mycelium cells,resulting in the outflow of cell contents and the hindering of normal biochemical process.

关 键 词：蜜环菌 链格孢霉菌 乙酸乙酯提取物 膜系统 

分 类 号：Q949.325[生物学—植物学] S432[农业科学—植物病理学]