根癌农杆菌介导的菌根真菌遗传转化研究进展  

Research Progress on Agrobacterium tumefaciens-Mediated Transformation System of Mycorrhizal Fungi

作　　者：邹嵘 马嘉楠 张凤[1] ZOU Rong;MA Jianan;ZHANG Feng(Institute of Innovation Ecology,State Key Laboratory of Grassland Agro-ecosystems of Lanzhou University Lanzhou 730000,China)

机构地区：[1]兰州大学草地农业生态系统国家重点实验室,生态学创新研究院,兰州730000

出　　处：《菌物研究》2021年第3期197-206,共10页Journal of Fungal Research

基　　金：兰州大学中央高校基本科研业务费专项资金资助项目(lzujbky-2019-ct01);国家自然科学青年科学基金项目(31901279);甘肃省自然科学基金项目(20JR5RA276)。

摘　　要：菌根真菌与寄主植物之间有着互利共生的关系,相互作用下会形成菌根结构。为了从基因水平上进行共生关系的研究,需要构建高效稳定的遗传转化体系。相比较传统的真菌遗传转化方法,根癌农杆菌Agrobacterium tumefaciens介导的转化方法具有适用范围广、转化效率高等特点,因此成为菌根真菌最理想的转化方法之一。通过探究影响根癌农杆菌遗传转化效率的因素以及所用的RNA干扰和过表达的克隆载体构建,总结出农杆菌转化外生菌根真菌的最适方案。在基因功能研究方面,归纳了已有的一些利用根癌农杆菌对不同类型菌根真菌进行遗传改造的实验结果,对兰科菌根真菌、杜鹃花菌根真菌、丛枝菌根真菌以及外生菌根真菌的基因功能研究进展进行总结。为菌根进行分子水平和遗传学方面的深入研究提供背景资料。The mutualism between mycorrhizal fungi and host plant would induce to form the mycorrhizae.For the research on symbiotic relationship at geneticlevel,we need to build the high efficient and stable genetic transformation system.Comparing with the traditional genetic transformation methods of fungi,Agrobacterium tumefaciens-mediated transformation is one of the most ideal way to produce transformants which has a wide range and high efficiency.In this review we explored the effects of the efficiency of A.tumefaciens-mediated transformation in various conditions and the construction of cloning vector about RNA interference and overexpression summarized the optimum method of A.tumefaciens transforming the ectomycorrhizal fungi.In the field of genetic function,generalized some results of different types of mycorrhizal fungi using the A.tumefaciens-mediated transformation to get the genetic modification and summarize the research progress of gene function about orchid mycorrhizal fungi,ericoid mycorrhizal fungi,arbuscular mycorrhizal fungi and ectomycorrhizal fungi.Therefore,it provided the background for further studied of mycorrhizae using molecular methods.

关 键 词：外生菌根真菌 农杆菌遗传转化 双色蜡蘑 

分 类 号：Q949.32[生物学—植物学]