藏族药榜嘎HPLC指纹图谱研究及其2种基原植物LC-MS/MS化学成分分析  

HPLC fingerprint of Tibetan medicinal plant ″Bangga″ and chemical components analysis of its two original plants by LC-MS/MS

作　　者：卢晨娜 杨悦[1] 白思宇 冯伟红[1] 梁曜华[1] 刘晓谦[1] 王智民[1] 李春[1] LU Chen-na;YANG Yue;BAI Si-yu;FENG Wei-hong;LIANG Yao-hua;LIU Xiao-qian;WANG Zhi-min;LI Chun(National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)

机构地区：[1]中国中医科学院中药研究所中药质量控制技术国家工程实验室,北京100700 [2]中国中医科学院广安门医院,北京100053

出　　处：《中国中药杂志》2021年第19期5027-5037,共11页China Journal of Chinese Materia Medica

基　　金：中央级公益性科研院所基本科研业务费专项(ZXKT17039)。

摘　　要：为全面认识藏族药榜嘎化学成分组成,该文建立了榜嘎药材HPLC指纹图谱分析方法并经LC-MS对其2种基原植物(船盔乌头与甘青乌头)的共有化学成分进行了推测。HPLC-DAD指纹图谱条件:Kromasil 100 C8色谱柱(4.6 mm×250 mm, 5μm),乙腈(A)-0.1%甲酸水(B)为流动相进行梯度洗脱,检测波长255 nm,流速1 mL·min^(-1),柱温35℃,进样量10μL。在此条件下记录17批榜嘎药材的色谱图,并将其导入《中药色谱指纹图谱相似度评价系统(2012版)》进行相似度评价。结果17批榜嘎药材HPLC图中共选出16个共有峰,以此16个共有峰为评价指标,17批样品的相似度介于0.702~0.966。各选择1批船盔乌头和1批甘青乌头药材进行LC-MS分析,从中共推测出74个共有成分,包括10个酚酸类、26个黄酮苷类和38个生物碱类化合物。该文建立的分析方法简单易行、分离度好、专属性强,可用于榜嘎药材的质量控制及其2种基原植物的鉴别。甘青乌头和船盔乌头在生物碱类成分组成种类和含量上类似,但二者黄酮类成分的含量上有显著差异。To obtain the chemical profile of Tibetan medicinal plant ″Bangga″, the present study established the HPLC fingerprint of ″Bangga″ and inferred common chemical constituents of its two original plants, Aconitum tanguticum and A. naviculare by LC-MS. The HPLC analysis was performed on a Kromasil 100 C8 column(4.6 mm×250 mm, 5 μm) with acetonitrile(A)-0.1% formic acid in water(B) as mobile phase in a gradient elution mode. Besides, the flow rate was set at 1 mL·min^(-1) and the column temperature was 35 ℃. The detection wavelength was set at 255 nm and the injection volume was 10 μL. Seventeen batches of ″Bangga″ samples were analyzed and the HPLC fingerprint was established under the above conditions. Similarity evaluation was performed using Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012). As a result, 16 common peaks were selec-ted and the similarity values of 17 batches of ″Bangga″ were in the range of 0.702-0.966. Furthermore, one batch of A. tanguticum and one batch of A. naviculare were analyzed by LC-MS/MS and 74 common compounds were inferred, including 10 phenolic acids, 26 flavonoids, and 38 alkaloids. The established method, with good separation and strong specificity, is simple and feasible, and can be used for the quality control of ″Bangga″ and identification of its two original plants. A. tanguticum and A. naviculare are similar in chemical composition and component content, but are quite different in the content of flavonoids.

关 键 词：榜嘎 HPLC指纹图谱 LC-MS 甘青乌头 船盔乌头 

分 类 号：R29[医药卫生—民族医学]