Gene abundances of AOA,AOB,and anammox controlled by groundwater chemistry of the Pearl River Delta,China  

作　　者：Kun Liu Xin Luo Jiu Jimmy Jiao Ji-dong Gu Ramon Aravena 

机构地区：[1]Department of Earth Sciences,Faculty of Science,The University of Hong Kong,Hong Kong 999077,China [2]Department of Ecology and Biodiversity,School of Biological Sciences,The University of Hong Kong,Hong Kong 999077,China [3]Department of Earth and Environmental Sciences,University of Waterloo,Waterloo N2L 3G1,Canada [4]China Institute of Geo-Environment Monitoring,China Geological Survey,Beijing 100081,China

出　　处：《China Geology》2021年第3期463-475,共13页中国地质（英文）

基　　金：This study was financially supported by the General Research Fund of the Research Grants Council,the Hong Kong Special Administrative Region,China(HKU 702612P and HKU 703010P).

摘　　要：Ammonia-oxidizing archaea(AOA),ammonia-oxidizing bacteria(AOB),and anaerobic ammonia-oxidation(anammox)bacteria are very important contributors to nitrogen cycling in natural environments.Functional gene abundances of these microbes were believed to be well relevant to N-cycling in groundwater systems,especially in the Pearl River Delta(PRD)groundwater with unique high intrinsic ammonia concentrations.In this research,20 sediment samples from two in the PRD were collected for porewater chemistry analysis and quantification of N-cycling related genes,including archaeal and bacterial amoA gene and anammox 16S ribosomal Ribonucleic Acid(rRNA)gene.Quantitative Polymerase Chain Reaction(qPCR)results showed that gene abundances of AOA,AOB,and anammox bacteria ranged from 3.13×10^(5)to 3.21×10^(7),1.83×10^(4)to 2.74×10^(6),and 9.27×10^(4)to 8.96×10^(6)copies/g in the sediment of the groundwater system,respectively.Anammox bacteria and AOA dominated in aquitards and aquifers,respectively,meanwhile,the aquitard-aquifer interfaces were demonstrated as ammonium-oxidizing hotspots in the aspect of gene numbers.Gene abundances of nitrifiers were analyzed with geochemistry profiles.Correlations between gene numbers and environmental variables indicated that the gene abundances were impacted by hydrogeological conditions,and microbial-derived ammonium loss was dominated by AOA in the northwest PRD and by anammox bacteria in the southeast PRD.

关 键 词：ANAMMOX Ammonium oxidizing archaea(AOA) Ammonium oxidation bacteria(AOB) AQUITARD Groundwater Hydrogeological survey engineering Pearl River Delta China 

分 类 号：P641.3[天文地球—地质矿产勘探]