正红树种子内生真菌Pestalotiopsis Foedans次生代谢产物及其生物活性研究  

Metabolites and Biological Activity of Pestalotiopsis Foedans,a Rhizophora Apiculata-Derived Endophytic Fungus

作　　者：王鸣慧 颜雅筠 龙腾腾[1] 江海洋 徐翠梅 刘延英 王守仓 Wang Minghui;Yan Yajun;Long Tengteng;Jiang Haiyang;Xu Cuimei;Liu Yanying;Wang Shoucang(Qingdao Huanghai University,Qingdao 266427;Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361000,China)

机构地区：[1]青岛黄海学院护理与健康学院,山东青岛266427 [2]自然资源部第三海洋研究所,福建厦门361000

出　　处：《广东化工》2021年第18期50-51,共2页Guangdong Chemical Industry

基　　金：青岛黄海学院校级课题(2019boshi03)。

摘　　要：对分离自正红树种子内生真菌污斑拟盘多毛孢(Pestalotiopsis foedans)进行了次生代谢产物及生物活性研究。通过多种分离纯化方法(常规硅胶柱层析、凝胶Sephadex LH-20柱层析、制备薄层层析等)和现代波谱学技术(紫外、核磁共振技术)分离鉴定了5个化合物结构,分别为:1,2-bis(3-indolyl)ethane(1),3-indolylethanol(2),adenosine(3),Uridine(4),Inosine(5)。对化合物进行卤虫致死活性测试,结果表明化合物1和5在浓度为100μg/mL时对卤虫的致死率分别为53.1%和36.7%。Cultivation of the endophytic fungus Pestalotiopsis foedans,which was isolated from the seed of Rhizophora apiculata sample,afforded five compounds.These compounds were isolated by repeated column chromatography on silica gel,RP-18,Sephadex LH-20,preparative thin layer chromatography as well as recrystallization.These structures were elucidated mainly based on the analysis of the UV,1D and 2D NMR as 1,2-bis(3-indolyl)ethane(1),3-indolylethanol(2),adenosine(3),Uridine(4),Inosine(5).In brine shrimp lethality activity assay,compounds 1 and 5 exhibited moderate brine shrimp lethality activity with lethality rate of 53.1% and 36.7%,at a concentration level of 100μg/mL,respectively.

关 键 词：正红树 污斑拟盘多毛孢 内生真菌 次生代谢产物 卤虫致死活性 

分 类 号：O629[理学—有机化学]