高效液相色谱法测定烟酰胺中烟酸的含量  

Determination of Nicotinic Acid in Nicotinamide by High Performance Liquid Chromatography

作　　者：刘红梅 张黎兴 Liu Hongmei;Zhang Lixing(Guangdong Qingsu Biological Technology Co.,Ltd.,Guangzhou Branch,Guangzhou 510800,China)

机构地区：[1]广东轻素生物科技有限公司广州分公司,广东广州510800

出　　处：《广东化工》2021年第18期175-176,183,共3页Guangdong Chemical Industry

摘　　要：烟酰胺是化妆中极为常用的一种皮肤调理剂,副产物烟酸是其主要的刺激来源,建本文建立了一种测定烟酰胺中的烟酸的高效液相色谱方法:5mmol庚烷磺酸钠水溶液-甲醇(体积比92︰8)为流动相、Agilent5TC-C18(2)高效液相色谱柱分离、检测波长为260nm、流速为1ml/min、外标法定量检测烟酸的含量。该方法的检出限为0.08 mg/kg,定量限为0.24 mg/kg,此方法操作简便,具有良好的灵敏度和重现性。Niacinamide is a very commonly used skin conditioner in makeup,and the by-product niacin is its main source of irritation.This article established a high performance liquid chromatography method for the determination of niacin:1% Sodium heptane sulfonate aqueous solution-methanol(volume ratio 92︰8)as mobile phase,ZORBBAX-C18 high performance liquid chromatography column separation,detection wavelength at 260 nm,flow rate at 1 mL/min,external standard method for quantitative detection of niacin content.The detection limit of this method is 0.08 mg/kg,and the limit of quantification is 0.24 mg/kg.This method is easy to operate and has good sensitivity and reproducibility.

关 键 词：烟酸 烟酰胺 高效液相色谱 化妆品 原料 

分 类 号：TQ[化学工程]