双水相系统提取槐米总黄酮的工艺研究  

Study of Total Flavonoid Extraction from Sophora japonica L.Using Aqueous Two-phase System

作　　者：张爱珍 侯巧芝[2] 霍柯柯 ZHANG Ai-zhen;HOU Qiao-zhi;HUO Ke-ke(Henan Advanced Geology and Mining College,Zhengzhou 451464,Henan,China;School of Medicine,Huanghe Science and Technology College,Zhengzhou 450063,Henan,China)

机构地区：[1]河南地矿职业学院,河南郑州451464 [2]黄河科技学院医学院,河南郑州450063

出　　处：《食品研究与开发》2021年第20期137-141,共5页Food Research and Development

摘　　要：采用超声辅助双水相系统进行槐米总黄酮的提取,以萃取率为指标,考察了聚乙二醇1000(polyethylene glycol 1000,PEG1000)质量分数、料液比、浸提温度和硫酸铵质量分数对总黄酮提取的影响。通过正交试验得出最佳提取工艺参数为聚乙二醇1000质量分数28%,硫酸铵质量分数10%,料液比1∶60(g/mL),浸提温度40℃。在此工艺条件下进行3次平行试验,总黄酮的萃取率为95.91%,得率为20.53 mg/g。该提取方法工艺稳定,结果重现性好。In the study,the extraction process of flavonoids from Sophora japonica L.was investigated using an ultrasound-assisted aqueous two-phase system.The effects of the polyethylene glycol 1000(PEG1000)concentration,solid-liquid ratio,extraction temperature,and ammonium sulfate concentration on the extraction ratio have been investigated by an orthogonal experimental method.The results showed that the optimal extraction conditions were a PEG 1000 and ammonium sulfate concentration of 28%and 10%,respectively,a solid-liquid ratio of 1∶60(g/mL),extraction temperature 40℃.Three parallel tests were carried out under these conditions.The extraction rate was 95.91%and the yield was 20.53 mg/g.The process was stable and the results were reproducible.

关 键 词：双水相系统 槐米 总黄酮 提取工艺 

分 类 号：R284.2[医药卫生—中药学]